Matthiesen Sherrill (zipperroom2)
Beta-glucosidase (EC 3.2.1.21) catalyzes the hydrolysis of cellobiose and cellooligosaccharides containing (1 → 4)-beta-glycosidic bonds to glucose, which is crucial in cellulosic ethanol production. In this study, Aspergillus versicolor, a novel highly productive beta-glucosidase strain, was first isolated from Camptotheca acuminata seeds. The highest beta-glucosidase activity with 812.86 U/mL was obtained by using the response surface methodology, and a 14.4-fold has increased compared to the control. The beta-glucosidase was then purified to homogeneity with recovery yield and specific activity of 25.98% and 499.15 U/mg, respectively. To enhance its stability and recyclability, the purified beta-glucosidase was first immobilized onto magnetic MnO2 by electrostatic adsorption. The immobilized materials were characterized by FR-IT, TEM and FE-SEM. Compared with the free beta-glucosidase, the immobilized enzyme exhibited enhanced thermal stability (1.5-fold raise in half-life at 50 °C), and reusability (holding over 60% activity after eight cycles), besides, the optimum pH has increased to 6.0. Substrate specificity research suggested that the enzyme had high hydrolytic activity on cellobiose. It also had a hydrolysis effect on (1 → 3) and (1 → 6)-beta-glycosidic linkages. Application trials in cellulose hydrolysis revealed that the immobilized enzyme was comparatively more effective. Our results suggested this novel immobilized beta-glucosidase makes a promising alternative for the cellulosic ethanol production.Three-dimensional (3D) printing using biocompatible materials is a novel technology having a great potential for fabricating precise 3D scaffolds for tissue engineering. Alginate hydrogel undergoes unstable swelling and degradation properties as well as suffers from poor cell adhesion due to the lack of cell binding domains. These limit its applications in tissue engineering. In this study, 3D-printed alginate scaffolds were coated by branch polyethylenimine (PEI) to overcome the limitation of alginate because the branch PEI is a cationic polymer with a large number of active N-H groups. The results indicated that surface modification of 3D-printed alginate scaffolds using an appropriate concentration of PEI potentially promoted the fibroblast cells functions in 3D-printed alginate scaffolds, increased cell adhesion, cell proliferation and cell spreading through providing a large amount of N-H groups and increasing hydrophilicity of the surface. The degradation rate of alginate was degraded by interaction between N-H groups in PEI and -COO- groups in alginate structure and followed by the formation strong barrier layer in the interface of alginate and PEI. Therefore, ALG-PEI scaffolds can be a good candidate for tissue engineering and wound dressing applications.Welan gum is widely used in food, concrete additives, and oil recovery. Here we changed the capsule form of Sphingomonas strains by knocked out the sortase gene (srtW). The obtained welan gum was mainly composed of mannose, glucose, rhamnose, and glucuronic acid at a molar ratio of 4.05.81.61, respectively. Meanwhile, the molecular weight of welan gum decreased sharply (about 68 kDa). Moreover, the low molecular weight (LMW) welan gum was characterized by FT-IR and NMR spectroscopy. The rheological results revealed that the LMW welan gum solution is a pseudoplastic fluid with a lower apparent viscosity. Furthermore, the oscillation test illustrated stable dynamic viscoelasticity within the temperature range of 5-68 °C and frequency range of 0.01-15 rad/s. To the best of our knowledge, this is the first report of LMW welan gum production and characterization. VIT2763 These results provide references for LMW welan gum applications, and likely applicable for other biopolymers production.Immunosuppressed patients are at increased risk of developing hospital-acquired fungal infections. The risk of fungal infection from construction is well established, but water
