Axelsen Villadsen (wrenquail1)

Over the past two decades, therapeutic alliance research has increasingly focused on understanding the process by which the alliance is ruptured and repaired. This paper is the first to explore how alliance rupture segments from psychotherapy sessions differ from non-rupture segments on key dimensions of the referential process. A sample of 27 psychotherapy sessions were scored using a measure designed to identify rupture from non-rupture segments. These segments were then scored for key linguistic dimensions of the referential process. During ruptures patients manifested a referential process marked by a decrease in emotional engagement, an increase in a measure of distancing, and an increase in negation as compared to non-rupture segments. Therapists show similar patterns but, in addition, manifest a language pattern that suggests that during ruptures, therapists are attempting to make sense of, and self-disclose, aspects of their inner experience. Implications for research and clinical work are explored.The photo-thermal lens technique is based on the measurement of the gradient temperature that is produced in an illuminated sample by laser beam as a result of non-radiative relaxation. The sensitivity of photo-thermal lens is higher than conventional absorption techniques, due to the direct measurement of absorbed energy. However, the main drawback of the photo-thermal lens technique is the inability to measure molecules that release their excess energy of absorption in the form of radiation relaxation. In fluorescence molecules, because their excess energy is released by radiation, the photo-thermal lens is insignificant. In this research, we aim to increase the photo-thermal lens sensitivity of the fluorescence molecules by selecting the appropriate acceptor for the fluorescence molecules as the donor. We investigated the effect of the presence of AuNPs on the photo-thermal lens of di-triazene (DTA) as fluorescence molecules. We observed that AuNPs enhanced the photo-thermal lens of DTA. The quantum yield of DTA was calculated to be 90.45%. The energy transfer from DTA to AuNPs occurs by fluorescence resonance energy transfer (FRET) mechanism. The FRET efficiency was 83.43%. In the presence of AuNPs, the photo-thermal lens of the DTA was amplified by four times more. The calibration curve of DTA is linear in the range of 0.1 to 110 ng/mL. The limited of detection (LOD) value of 0.08 ng/mL is calculated. We investigate the possibility to exploit high-field MRI to acquire 3D images of Purkinje network which plays a crucial role in cardiac function. Since Purkinje fibers (PF) have a distinct cellular structure and are surrounded by connective tissue, we investigated conventional contrast mechanisms along with the magnetization transfer (MT) imaging technique to improve image contrast between ventricular structures of differing macromolecular content. Three fixed porcine ventricular samples were used with free-running PFs on the endocardium. GSK461364A T1, T2*, T2, and M0 were evaluated on 2D slices for each sample at 9.4T. MT parameters were optimized using hard pulses with different amplitudes, offset frequencies and durations. The cardiac structure was assessed through 2D and 3D T1w images with isotropic resolutions of 150µm. Histology, immunofluorescence, and qPCR were performed to analyze collagen contents of cardiac tissue and PF. An MT preparation module of 350ms duration inserted into the sequence with a B1 = 10 µT and frequency offset = 3000Hz showed the best contrast, approximately 0.4 between PFs and myocardium. Magnetization transfer ratio (MTR) appeared higher in the cardiac tissue (MTR = 44.7 ± 3.5%) than in the PFs (MTR = 25.2 ± 6.3%). MT significantly improves contrast between PFs and ventricular myocardium and appears promising for imaging the 3D architecture of the Purkinje network. MT significantly improves contrast between PFs and ventricular myocardium an