Chan Hjelm (virgooak26)

Our results demonstrated that 5 μM SFN improved all parameters of sperm including viability (P less then 0.001), motility, and morphology (P less then 0.05) after the freeze-thaw process. Furthermore, SFN reduced the levels of intracellular hydrogen peroxide (P less then 0.01) and superoxide anion (P less then 0.05). Also, SFN significantly increased the percentage of viable sperm cells with the intact plasma membrane (P less then 0.001) and decreased the level of lipid peroxidation after the freeze-thaw process (P less then 0.01).Our findings showed that spermatozoa treatment with 5 μM SFN before the freeze-thaw process has protective effects against oxidative stress and could decrease the detrimental effects of this process on sperm quality.The therapeutic effects of cryotherapy on skin and subcutaneous tumors in dogs were retrospectively studied in 20 dogs with 37 tumor lesions, of which 30 were benign and seven were malignant. Our results showed that during follow-up, 94.5% of lesions were completely exfoliated, without relapse or metastasis (mean time = 245.7 days). To investigate the effects of cryotherapy, we compared histopathological observations and microstructural changes in healthy tissues and tumor tissues, before and after cryotherapy. After cryotherapy, both normal skin and tumor tissue exhibited edema and hyperemia, with inflammatory cell infiltration. The cell nuclei exhibited pyknosis, disintegration and necrosis, and tight junctions were decreased in size. Cell morphology was varied, along with fragmented cell nuclear envelopes, crenulated nuclei and indistinct and necrotic intracellular organelles. Vacuoles were apparent in the cytoplasm and intercellular desmosomes were absent. These observations suggested that cryosurgery inhibited skin and subcutaneous tumors via cold-induced injury to cells, and cellular microenvironment changes induced by apoptosis. The results suggested that cryosurgery prevented skin and subcutaneous tumors via cold-induced injury to cells, and cellular microenvironment changes induced by apoptosis. We believe these data will provide general cryotherapy guidance to scientists and veterinary surgeons.Cryopreservation of gametes, embryos and larvae of marine invertebrates has been investigated in many studies throughout the years. There are many favorable studies on sperm cryopreservation but oocytes are still under research as no successful results have been sustainably obtained for this type of cells. The preservation of both maternal and paternal gametes separately would provide a reliable source of genetic material for their application to conservation, aquaculture and fundamental research. Unfortunately to date, it has not been possible to cryopreserve eggs from marine organisms. The aim of this review is to go over the factors that have been historically considered as obstacles for oocyte cryopreservation in aquatic organisms and discern those that may specifically apply to eggs of the sea urchin Paracentrotus lividus.In swine, the use of frozen-thawed boar sperm for artificial insemination remains a suboptimal reproductive technology. Among the negative effects of cryopreservation on sperm cells, it is worth highlighting that cryopreservation causes irreversible alterations in motility and components of the sperm membrane as a result of dramatic changes in temperature (cooling/freezing curve) and osmolality. In addition, freeze-thawing may induce oxidative stress and increase the generation of reactive oxygen species (ROS) and nitrogen reactive species (RNS). While boar sperm cryopreservation has been reported to increase lipid peroxidation and the intracellular levels of hydrogen peroxide, less research on its impact on RNS has been conducted. Furthermore, previous studies have investigated the effects of supplementing cryopreservation media with antioxidants to counteract the deleterious effects of ROS and RNS. Antioxidants of synthetic origin or natural extracts have been u