Thyssen Alexander (traystate31)

nce was achieved for the prepared membranes even after four adsorption/regeneration cycles in the continuous UF experiments, demonstrating the feasibility and effectiveness of the prepared adsorptive nanofiber membranes for the removal of heavy metal ions.Thermus thermophilus laccase belongs to the sub-class of multicopper oxidases that is activated by the extra binding of copper to a methionine-rich domain allowing an electron pathway from the substrate to the conventional first electron acceptor, the T1 Cu. In this work, two key amino acid residues in the 1st and 2nd coordination spheres of T1 Cu are mutated in view of tuning their redox potential and investigating their influence on copper-related activity. Evolution of the kinetic parameters after copper addition highlights that both mutations play a key role influencing the enzymatic activity in distinct unexpected ways. These results clearly indicate that the methionine rich domain is not the only actor in the cuprous oxidase activity of CueO-like enzymes.High quality genome is of great significance for the mining of biological information resources of species. Up to now, the genomic information of several important economic flatfishes has been well explained. All these fishes are eyes on left side-type, and no high-quality genome of eyes on right side-type species has been reported. In this study, we applied a combined strategy involving stLFR and Hi-C technologies to generate sequencing data for constructing the chromosomal genome of Verasper variegates, which belongs to Pleuronectidae with characteristic of eyes on right side. The size of genome of V. variegatus is 556 Mb. More than 97.2% of BUSCO genes were detected, and N50 lengths of the contigs and scaffolds reached 79.8 Kb and 23.8 Mb, respectively, demonstrating the outstanding completeness and sequence continuity of the genome. A total of 22,199 protein-coding genes were predicted in the assembled genome, and more than 95% of those genes could be functionally annotated. Meanwhile, the genomic collinearity, gene family and phylogenetic analyses of similar species in Pleuronectiformes were also investigated and portrayed for metamorphosis and benthic adaptation. Sex related genes mapping has also been achieved at the chromosome level. This study is the first chromosomal level genome of a Pleuronectidae fish (V. variegatus). The chromosomal genome assembly constructed in this work will not only be valuable for conservation and aquaculture studies of the V. variegatus but will also be of general interest in the phylogenetic and taxonomic studies of Pleuronectiformes.Laser-Ultrasonics Testing (LUT) is a Non-Destructive Technique (NDT) with good potential for application in the railway sector, nevertheless this technique is not yet used in practice because there are some practical difficulties to overcome. The possibility of measuring on a complete wheelset by bypassing the keying of the wheel will allow drastically reducing the inspection time but it has not yet been demonstrated. In fact, the attenuation of the signal in the path makes complex the interpretation of the generated waves. This paper aims at illustrating how the combination of simulated and experimental data allows to optimize the test setup for having output data of unambiguous interpretation. The main innovations presented in this paper are (i) the possibility to work with low energy waves in the thermoelastic-ablative limit while maintaining satisfactory contrast levels for the purpose of defect detection and (ii) the implementation of a complete Finite Element Model (FEM) including the generation and propagation of waves in the solid domain and the propagation in air. This last step has not considered before in previous papers. The model allows to define the optimal experimental conditions to have a measured signal with an adequate signal-to-noise ratio (SNR > 6 dB) and to define an experimental procedure for defect detection reliable and comp