Ashworth Penn (tirerobert08)

The most prominently difference between the SERS spectrum of healthy brain tissue and that of gliomas at different grades is the reduction in quotient of two characteristic peaks at 653 and 724 cm-1. Furthermore, healthy brain tissue and Grade II gliomas as low grade gliomas as well as Grade III and Grade IV as high-grade gliomas can be clearly distinguished by three-dimensional PCA. Preliminary results indicate that the SERS spectra based on AgNPs@AgNR substrates can be applied for a rapid identification owing to its simple preparation of specimen and high-speed spectral acquirement.This critical Review covers the literature reports on analysis of different types of solid samples by the synchronous fluorescence spectroscopy (SFS) and its varieties, which include synchronous phosphorescence spectroscopy and synchronous luminescence spectroscopy, in the three decades (1990-2019). Both the qualitative and quantitative spectroscopic analysis is described for a wide range of specimens. Their physical forms and chemical composition include a) organic and inorganic analytes pre-concentrated from solution on matrices (beads, membranes, filters, disks, paper), b) natural and synthetic multi-component specimens of complex composition (biological tissues, soil, polymers) and c) inorganic and coordination compounds including porous materials and particularly metal-organic frameworks (MOFs). The comparison with the data obtained by "conventional" optical emission spectroscopy and other analytical techniques (when available) is presented. The specific advantages of the high-resolution varieties of the method, the first- and second-derivative solid-state synchronous fluorescence, luminescence, and phosphorescence spectroscopy are described. An attention is also paid to practical conditions of the typical tests, and the relevant experimental setups. The impetus is on the emerging capabilities of this highly promising method e.g. in-situ monitoring of chemical reactions, in-vivo diagnostics, surface reactions, and detection of the adsorbate. The existing challenges are analyzed, and the unexplored application "niches" to further develop this and the related analytical methods are revealed. 145 references, 9 Tables, 17 Figures and 1 Scheme.A novel HBT-based fluorescent dye HBTM, which exhibited long wavelength emission (~600 nm) and large Stokes shift (~203 nm) due to the intrinsic mechanism of ESIPT coupled ICT process, was reasonably designed and synthesized by conjugating neutral pyrimidine moiety as the electron acceptor to 2-(benzo[d]thiazol-2-yl)-4-methylphenol scaffold. Fluorescence emission of HBTM showed less significant spectral dependency on solvents nature, delivering excellent anti-hypochromatic properties, and notably enhanced quantum yield (Φ = 25.5%) in water system was obtained. Furthermore, a "Turn-On" fluorescent probe HBTMP was developed for the detection of NQO1 by masking the hydroxyl group of HBTM with quinone propionic acid (QPA) as the sensing group. Probe HBTMP displayed a highly sensitive and selective response to NQO1 with a linear relationship in the range of 60-180 ng/mL and low detection limit of 1.6 ng/mL, and was successfully applied in detecting endogenous NQO1 in living cancer cells.Understanding lysosome-related physiology needs specific lysosome probes to track the biological processes of lysosome in living cells. Here, we report an azacyclo-modified fluorescent probe that has a large Stokes shift, good photostability and negligible cytotoxicity for highly specific labeling of lysosome and autolysosome in living cells. The probes with different kinds of azacyclo groups on parent dye dansyl are screened to show that dansyl-cycleanine (DNS-C) with four nitrogen atoms possesses the best lysosome-localized ability. And DNS-C as a universal tracker exhibits excellent ability for lysosome labeling in different cell lines with high overlap coefficients (≥0.90). Different from a commercially available LysoTracker