Otte Salinas (tablegarage1)

An in-depth investigation into the anti-inflammatory effects of U. cannabina, both in vitro and in vivo, including the underlying mechanisms and its phytochemical profile, constitutes this study. Utilizing ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) for chemical profiling, we evaluated the anti-inflammatory effects of U. cannabina water extract on lipopolysaccharide-stimulated RAW2647 macrophages and rat paw edema. U. cannabina water extract's treatment resulted in a statistically significant (p<0.05) reduction in the secretion and mRNA expression of various inflammatory factors within LPS-induced RAW2647 cells. Rat paw edema, triggered by carrageenan, and inflammatory markers were markedly lowered by *Cannabis* tincture water extract (p<0.005). Tentatively, 31 compounds, consisting largely of organic acids, were identified by cross-referencing their mass spectrum profiles with those stored in a mass spectra database. In both laboratory and live animal studies, this research illustrated that U. cannabina water extract has an anti-inflammatory effect, this effect being attributable to its capacity to modulate the activity of inflammatory cytokines. U. cannabina's active compounds are validated by the results as possessing potent anti-inflammatory properties. The anti-inflammatory impact of U. cannabina water extract, demonstrated in both in vitro and in vivo models, stems from its modulation of inflammatory cytokine activity. U. cannabina's ability to produce active compounds possessing anti-inflammatory properties is firmly established by the results obtained. In Korean traditional herbal medicine, Dohongsammul-tang (DH) is a treatment for the symptoms resulting from blood leakage. This compound is effective in preventing cardiovascular diseases, achieved through the activation of blood circulation to resolve blood stasis. From the perspective of its medicinal characteristics, the DH has discovered the potential of relieving cardiac hypertrophy. In order to verify the pharmacological impact of DH on cardiovascular health and to highlight its concurrent enhancement of renal function, this study was conducted. In addition, the aim of this research is to discover fresh potential functionalities beyond the established medicinal benefits of DH, a traditional medicine. Cardiovascular disease often manifests as cardiac hypertrophy, characterized by a transformation in the heart's morphology as a consequence of pressure overload. It has been observed that an increase in the quantity of myofibrils directly leads to a thickening of the heart muscle, which can result in hypertension. Accordingly, curbing cardiac hypertrophy might be a primary contributor to decreasing the rates of morbidity, mortality, and heart failure that are consequences of cardiovascular disease. Consequently, this investigation sought to determine if the traditionally employed DH treatment could enhance the recovery from and mitigate the cardiac injury and fibrosis associated with cardiac hypertrophy. Dohongsamul-tang, a preparation of 6 herbal ingredients, was brewed using 4 liters of distilled water for 2 hours. The dohongsamul-tang mixture, subjected to centrifugation at 3000 rpm for 10 minutes, was concentrated afterwards. Extraction of the concentrated dohongsamul-tang, freeze-dried and stored at 70°C, was performed. The dohongsamul-tang powder was orally ingested following its dilution in distilled water. The study employed surgical ligation of the transverse aortic arch, connected to the left ventricle, to the size of a 27G needle in order to induce pressure overload. Cardiac hypertrophy and heart remodeling, resulting from the induction and maintenance of the process, persisted for eight weeks. Using a 10-week regimen of oral propranolol and dohongsamul-tang, researchers investigated the impact of these treatments on cardiac hypertrophy induced by transverse aortic constriction (T