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Improved experimental conditions led to the successful separation of ibrutinib enantiomers, requiring only 42 minutes of analysis time and demonstrating an enantioresolution of 15. The addition of fifteen CILs impacted enantioresolution, resulting in generally longer analysis times and improved enantioresolution. The combination of S, CD, and [TMA][L-Lys] proved effective in separating ibrutinib enantiomers, completing the process in 81 minutes and delivering an enantioresolution value of 33, replicating the results obtained using a single CD system under comparable experimental conditions. S-ibrutinib, the enantiomeric impurity, exhibited the fastest migration rate using both the single chiral detector and the combined chiral detector/chiral separation column methods, aligning with the best-case scenario for chromatographic analysis. According to the International Council on Harmonization, chiral methods facilitated the detection of enantiomeric impurities, attaining a level of 0.1%. Having defined the analytical attributes of both chiral methods, they were subsequently employed to determine the enantiomeric composition of ibrutinib within a hospital-grade pharmaceutical formulation, marketed as the pure R-enantiomer, along with assessing the stability and ecotoxicological impact of racemic ibrutinib and its R-enantiomer on Daphnia magna. Innovative methodologies facilitated the first rapid chiral quantitation of ibrutinib in both abiotic and biotic matrices. Utilizing triple quadrupole inductively coupled plasma mass spectrometry (ICP-MS/MS), an analytical method was developed to simultaneously detect 237Np, 239Pu, and 240Pu in environmental samples at levels as low as femtograms. OXPHOS signaling The consistent behavior of Np and Pu, achieved through the careful control of their valence states during the entire separation procedure using the single extraction chromatographic column (TK200), facilitated the reliable employment of 242Pu as a chemical yield tracer for 237Np, 239Pu, and 240Pu. A high decontamination factor, 32,107, was achieved for the most interfering element, uranium, during the chemical separation process. In the measurement of plutonium isotopes, the interferences from 238U1H+ and the problematic peak tailing of 238U+ were effectively removed using 75 mL/min He-11 mL/min CO2 as reaction gases in the octupole collision/reaction cell, combined with sequential quadrupole mass separation within the ICP-MS/MS system. Reaction gases enabled the interference of 238U1H+ to be lowered to 10-6 and the peak tailing of 238U+ to be reduced to 10-10. This represents a remarkable three orders of magnitude performance enhancement compared to methods lacking reaction gases. In samples, the developed method enables the precise determination of 237Np, 239Pu, and 240Pu at femtogram levels, given the U/Np and U/Pu atom ratios reaching up to 10^17 and 10^12, respectively. The developed method's validity was established through the analysis of standard reference materials and spiked soil samples. The facile synthesis of two-dimensional iron metal-organic framework nanosheets (2D Fe MOF) at room temperature involved the simple stirring of iron salts and the terephthalic acid ligand within a mixed solution containing triethylamine. Detailed characterization of the morphology and structure was performed using TEM, AFM, XPS, and TEM element mapping techniques. A study of the peroxidase-mimicking activity was carried out using H₂O₂ and 3,3',5,5'-tetramethylbenzidine as substrates. The dissociation constant (Km) of the 2D Fe MOF for H2O2 was 0.002 mM, and its catalytic rate (Vmax) was 2.08 x 10^-8 M per second. Through a cascade reaction system involving xanthine oxidase and 2D Fe MOF, a visual technique for detecting hypoxanthine (Hx) was created, enabling evaluation of aquatic product freshness. The method demonstrated a wide linear range (50-5000 M), a low detection limit of 329 M, high accuracy (9478-9985%
