Elliott Linnet (subwayhair49)
Chemical modification of biological materials is indispensable for enrichment of phosphorylated peptides. In this work, we synthesized a biomimetic honeycombed affinity chromatography (IMAC) adsorbent by preparing Crosslinked Chitosan, chelating aminomethyl phosphate decorated with Ti (IV) cation. The as-prepared CTSM@AMPA-Ti4+ composites with stable structure, low steric hindrance, and high Ti4+ loading amount were used as a promising adsorbent for enrichment of phosphopeptides. CTSM@AMPA-Ti4+ showed extremely high sensitivity (0.4 fmol) and selectivity at a low composition molar ratio of β-casein/BSA (11000). What's more, it can keep its performance in the case that used to capture phosphorylated peptides from standard protein ten times or soaking in the acid/base solution for a long time. In addition, CTSM@AMPA-Ti4+ successfully captured 35 phosphorylated peptides from human saliva. This study offers a way about diversiform functionalization of CTSM in phosphoproteome analysis and disease research.The present research is focused on the preliminary evaluation, in particular in relation to the advisable operational conditions, of a novel low duty cycle flow modulator. In such a respect, a fast comprehensive two-dimensional gas chromatography-mass spectrometry method is herein proposed. Applications on a C7-C30 series of alkanes, 64 fragrance allergens (plus 2 internal standards), and 5 perfumes, were carried out by using two different column sets, low-polarity + medium-polarity and low-polarity + low-polarity. In both cases, the first column was of dimensions 10 m × 0.25 mm ID × 0.25 µm df, while the second one was of dimensions 1 m × 0.10 mm ID × 0.10 µm df. A modulation period of 700 ms, with a re-injection period of 80 ms, was used in order to obtain a higher duty cycle (measured to be approx. 0.04). Absolute quantification of the allergens was carried out by using two internal standards, namely 1,4-dibromobenzene and 4,4'-dibromobiphenyl. In terms of limits of quantification the instrumental response was characterized by a wide variability, ranging between 9 ppb and 5.4 ppm for both column sets. A total number of 97 fragrance allergens were identified and quantified in five commercial perfumes.Anions play crucial roles in the sustenance of life on earth in many ways. Selective detection of specific anions is important in developing new diagnostic tools and therapeutics. A pH-sensitive & selective benzimidazole-based fluorescent sensor has been developed for rapid detection of carbonate ions which can detect carbonate ions in low nanomolar concentrations. NMR based experiments indicate direct interaction of benzimidazole imino protons with the carbonate ions leading to 11 ligand carbonate ion complexation events. This is one of the first reports of benzimidazole sensing carbonate ions with high selectivity which may have implications in disease prevention and toxicity assessment.Hepatitis B is a contagious liver disorder caused by hepatitis B virus and if not treated at an early stage, it becomes chronic and results in liver cirrhosis and hepatocellular carcinoma which can even lead to death. In present study, surface-enhanced Raman spectroscopy (SERS) is employed for the analysis of polymerase chain reaction (PCR) products of DNA extracted from hepatitis B virus (HBV) infected patients in comparison with healthy individuals. SERS spectral features are identified which are solely present in the HBV positive samples and consistently increase in intensities with increase in viral load which can be considered as a SERS spectral marker for HBV infection. For sake of understanding, these various levels of viral loads in this study are classified as low (1-1000 IU), medium (1000-10,000 IU), high (above 10,000 IU) and negative control (>1). In order to explore the efficiency of SERS for discrimination of SERS spectral datasets of different samples of varying viral loads and healthy individuals, principal component analys
