Hooper Smedegaard (silkland0)

A simple and rapid reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the detection of SARS-CoV-2. The RT-LAMP assay was highly specific for SARS-CoV-2 and was able to detect one copy of transcribed SARS-CoV-2 RNA within 24 minutes. Assay validation performed using 50 positive and 32 negative clinical samples showed 100% sensitivity and specificity. The RT-LAMP would be valuable for clinical diagnosis and epidemiological surveillance of SARS-CoV-2 infection in resource-limited areas as it does not require the use of sophisticated and costly equipment. It is believed that radiotherapy has important effects on oxidant/antioxidant systems. Oxidative stress occurs when the balance between oxidant formation and antioxidant defense is disrupted in favour of oxidants. The aim of this study was to determine the biochemical changes in saliva pre- and post-radiotherapy in head-neck radiotherapy patients and to find out the effects of radiation on glutathione (GSH), glutathione peroxidase (GSH-Px), and Malondialdehyde (MDA) levels in saliva. This study included 16 patients undergoing head-neck radiotherapy in the Atatürk University Research Hospital. The levels of GSH, GSH-Px, and MDA were measured in saliva samples taken from the patients pre- and post-radiotherapy. The same biochemical parameters were also measured in saliva samples from 30 healthy individuals who did not undergo head-neck radiotherapy. The data obtained were analysed using the paired t-test and the Mann-Whitney U test. When the levels of GSH (p > 0.05), GSH-Px (p > 0.05), and MDA (p < 0.05) in saliva were compared pre- and post-radiotherapy in the patient group, the only significant increase was detected in MDA level post-radiotherapy. When the pre- and post-radiotherapy levels of saliva GSH (p < 0.01, p < 0.001, respectively), GSH-Px (p > 0.05, p < 0.05, respectively), and MDA (p < 0.01, p < 0.001, respectively) were compared with those of the control group, it was revealed that the GSH level significantly lower and the MDA level was significantly higher in both pre- and post-radiotherapy compared to the control group. Also, only the post-radiotherapy saliva GSH-Px level was found to be significantly lower than the control group. These findings show that the changes in saliva GSH, GSH-Px and MDA levels in patients with head-neck malignity intensified due to radiation. These findings show that the changes in saliva GSH, GSH-Px and MDA levels in patients with head-neck malignity intensified due to radiation. To evaluate diagnostic efficacy of the apparent diffusion coefficient measurements from tumor (ADCt) and tumor circumference hyperintensities (ADCtch) in different types of malignant intra-axial brain tumors. Between April 2013 and June 2017, 125 patients (52 females (41.6%) and 73 males (58.4%); mean age 53 years, age range 14-81 years), who underwent diffusion-weighted imaging (DWI) with intracranial mass, were retrospectively evaluated. The mean ADCt and ADCtch values and ratios were measured. Of the 125 patients, 22 (17.6%) had a low-grade glioma (LGG), 55 (44%) had a high-grade glioma (HGG), 32 (25.6%) had metastasis, and 16 (12.8%) had lymphoma diagnosis. There was a statistically significant difference in LGG and HGG in terms of mean ADCt and mean ADCtch values, and ratios. ADCtch values and ratios showed a statistically significant difference in the differentiation of HGG and metastasis and in the differentiation of HGG and lymphoma. According to ROC curve analysis, a cut-off value of 1.49 × 10−3 mm2/s for the mean ADCtch value generated the best combination of 70% sensitivity and 71% specificity for differentiation of HGGs and metastasis. The mean ADCtch value had the highest statistical predictive value for differentiation of HGGs and lymphoma with a sensitivity of 78% and a specificity of 76% for the optima