Mathews Tate (serverflag0)

hed by practices despite the regularly updated, blanket information provided by the BOS. Communication may have been delivered by a different means during the pandemic which this study did not account for. In addition, the sampling method may not have identified all practices within the London region, however the sample size seems appropriate to draw meaningful conclusions. The checklist created should help improve the delivery of future information.The study was conducted to assess the present status of knowledge, attitudes and practices (KAP) concerning the food safety and hygiene issues among fish farmers and food handlers in Noakhali, Bangladesh. Data were collected from 100 respondents (50 fish farmers and 50 food handlers) through face to face interviews. All the respondents agreed that training on hygiene practices was necessary and they were interested to attend training in their spare time. Food handlers (88%) agreed that raw foods must be kept separate from prepared foods and 68% of them believed that improper preservation of foods is harmful to health. About 52% of farmers used a face mask when spreading insecticides, fertilizers, and chemicals. Food handlers were wearing protective clothing (32%) and masks (12%) when touching and distributing unwrapped foods, however no handlers used a cap. A significant strong correlation coefficient (rs) was found between knowledge with attitudes, knowledge with practices and attitudes with practices of 0.73-0.99 among the three domains for fish farmers and food handlers. The correlation among the three levels was satisfactory, however, some food safety concepts and practices regarding hygiene were still insufficient. Therefore, an effective and appropriate training program on food safety and hygiene should be launched in a holistic approach for all fish farmers and food handlers to increase awareness and ensure safe food for consumers with a view to reducing the possibility of disease outbreak.Piper capense Linn is a plant used in Cameroon to treat cancer and several other diseases such as urinary tract disorder, fever, stomach-ache and to improve appetite. The methanol extract of Piper capense has been reported for its antiproliferative activity towards several human cancer cell lines. The aim of this work was to evaluate the acute and subchronic oral toxicities of a methanol extract from P. capense fruits on rats. The acute oral toxicity assay was carried out by administration of a single dose of 5000 mg/kg body weight of methanol extract of the Piper capense to five female rats, after which the behavior of the animals and the number of deaths were noted after 48 h. The animals were then kept for observation for 14 days. On the 15th day, the rats were sacrificed and macroscopic observation of the organs was made. Concerning the subchronic toxicity study, the rats composed of males and females received three doses (250, 500 and 1000 mg/kg body weight/day) for a period of 28 days by oral gavage. Gee liver and kidney organs at a dose of 1000 mg/kg body weight/day. Finally, the methanol extract of Piper capense fruits is safe at lower doses, but could cause some damages at doses as high as 1000 mg/kg body weight/day. Consequently, it should be taken with caution when used in therapy.The wide scale application of pectinase is generally hindered by high cost of the enzyme production. In this work, a two dimensional strategy was adopted to reduce cost of pectinase production by Geotrichum candidum AA15. The strain was immobilized in alginate beads. The optimum concentration for bead formation was found to be 3.5% of sodium alginate (NA) with 4% calcium chloride (CaCl2). Such immobilized cells retained the ability to produce 0.115 IU mL-1 of pectinase for up to 6th production cycle in citrus pectin containing medium while free cells produced only 0.046 IU mL-1 of pectinase. For the cultivation of immobilized cells on orange peels (OP), a combination of 4.5% NA and 4% CaCl2 was found