Vogel Farmer (seederdeal36)
Engineered colloidal nanoparticles show great promise in biomedical applications. While much of the work of assessing nanoparticle impact on living systems has been focused on the direct interactions of nanoparticles with cells/organisms, indirect effects via the extracellular matrix have been observed and may provide deeper insight into nanoparticle fate and effects in living systems. In particular, the large surface area of colloidal nanoparticles may sequester molecules from the biological milieu, make these molecules less bioavailable, and therefore function indirectly as "molecular knockouts" to exert effects at the cellular level and beyond. In this paper, the hypothesis that molecules that control cellular behavior (in this case, chemoattract molecules that promote migration of a human monocytic cell line, THP-1) will be less bioavailable in the presence of appropriately functionalized nanoparticles, and therefore the cellular behavior will be altered, was investigated. Three-dimensional chemotaxis assays for the characterization and comparison of THP-1 cell migration upon exposure to a gradient of monocyte chemoattractant protein-1 (MCP-1), with and without gold nanoparticles with four different surface chemistries, were performed. By time-lapse microscopy, characteristic parameters for chemotaxis, along with velocity and directionality of the cells, were quantified. Anionic poly(sodium 4-styrenesulfonate)-coated gold nanoparticles were found to significantly reduce THP-1 chemotaxis. Enzyme-linked immunosorbent assay results show adsorption of MCP-1 on the poly(sodium 4-styrenesulfonate)-coated gold nanoparticle surface, supporting the hypothesis that adsorption of chemoattractants to nanoparticle surfaces interferes with chemotaxis. Free anionic sulfonated polyelectrolytes also interfered with cell migrational behavior, showing that nanoparticles can also act as carriers of chemotactic-interfering molecules.A zirconium-based metal-organic framework (MOF) was successfully constructed via solvothermal assembly of a triphenylamine-based tricarboxylate ligand and Zr(IV) salt, the structure simulation of which revealed that it possesses a two-dimensional layered framework with a relatively rare dodecnuclear Zr12 cluster as the inorganic building unit. The inherent photo-responsive property derived from the incorporated photochromic triphenylamine groups combined with its high stability makes the constructed MOF an efficient heterogeneous photocatalyst for the oxidation of sulfides, which is a fundamentally important reaction type in both environmental and pharmaceutical industries. The photocatalytic activity of the constructed MOF was first investigated under various conditions with thioanisole as a representative sulfide substrate. The MOF exhibited both high efficiency and selectivity on aerobic oxidation of thioanisole in methanol utilizing molecular oxygen in air as the oxidant under blue light irradiation for 10 h. Its high photocatalytic performance was also observed when extending the sulfide substrate to diverse thioanisole derivatives and even a sulfur-containing nerve agent simulant (2-chloroethyl ethyl sulfide). The high photocatalytic efficiency and selectivity to a broad set of sulfide substrates make the triphenylamine-incorporating zirconium-based MOF a highly promising heterogeneous photocatalyst.The technologically important frequency range for the application of electrostrictors and piezoelectrics is tens of Hz to tens of kHz. Sm3+- and Gd3+-doped ceria ceramics, excellent intermediate-temperature ion conductors, have been shown to exhibit very large electrostriction below 1 Hz. Why this is so is still not understood. While optimal design of ceria-based devices requires an in-depth understanding of their mechanical and electromechanical properties, systematic investigation of the influence of dopant size on frequency response is lacking. In this report, the mechanical and electromechanical properties o
