Quinlan Willadsen (sackshark0)

Changing rules to promote scoring through more 'high-risk' play has become common in team sports. Australia's national netball league (i.e. Suncorp Super Netball) has recently taken this approach-introducing a two-point shooting rule. Teams will be awarded two-points for shots made from an 'outer circle' 3.0m-4.9m from the goal in the final five minutes of quarters. We sought to answer a series of questions regarding the implementation and potential strategies surrounding the two-point rule in Suncorp Super Netball. We used video coded data from the 2018 Suncorp Super Netball season to identify the total number of made and missed shots from different distances across the season. We also used shooting statistics from recent Fast5 Netball World Series (a competition with a two-point shooting rule already in place) as a comparator. The reward of two-points is relatively well-aligned to the relative risk of missing shots from the proposed outer versus inner circle (2.22 [1.98, 2.48 95% CIs]) based on existing shooting data from Suncorp Super Netball teams. We found that the relative risk of missing shots from 'long-' (i.e. 3.5m-4.0m) versus 'mid-range' (i.e. 3.0-3.5m) was only slightly elevated (1.52 [1.21, 1.86 95% CIs])-suggesting teams should favour long- over mid-range shots when the two-point shot is available. Based on the typical number of shots a team receives in a five-minute period, we found that teams may be able to score ~3.51 extra points per quarter when taking all versus no-shots from the two-point outer circle. Analysis of the Fast5 versus Suncorp Super Netball data did, however, reveal that shooting accuracy from long-range may decrease when a two-point shot is available. Teams may need to consider situational factors (e.g. altered opposition defensive strategies) when developing their shooting strategy for taking advantage of the two-point shot.Sensorineural hearing loss is one of the most common disabilities worldwide. Such prevalence necessitates effective tools for studying the molecular workings of cochlear cells. One prominent and effective vector for expressing genes of interest in research models is adeno-associated virus (AAV). However, AAV efficacy in transducing cochlear cells can vary for a number of reasons including serotype, species, and methodology, and oftentimes requires high multiplicity of infection which can damage the sensory cells. Reports in other systems suggest multiple approaches can be used to enhance AAV transduction including self-complementary vector design and pharmacological inhibition of degradation. Here we produced AAV to drive green fluorescent protein (GFP) expression in explanted neonatal mouse cochleae. Treatment with eeyarestatin I, tyrphostin 23, or lipofectamine 2000 did not result in increased transduction, however, self-complementary vector design resulted in significantly more GFP positive cells when compared to single-stranded controls. Similarly, self-complementary AAV2 vectors demonstrated enhanced transduction efficiency compared to single stranded AAV2 when injected via the posterior semicircular canal, in vivo. Self-complementary vectors for AAV1, 8, and 9 serotypes also demonstrated robust GFP transduction in cochlear cells in vivo, though these were not directly compared to single stranded vectors. These findings suggest that second-strand synthesis may be a rate limiting step in AAV transduction of cochlear tissues and that self-complementary AAV can be used to effectively target large numbers of cochlear cells in vitro and in vivo.The objective of this study was to evaluate the effects of the association of cottonseed processing method with chitosan on carcass traits and meat quality of lambs finished in feedlot. Eighty lambs with an average body weight of 20.6 kg, with 04 months of age, were distributed in a completely randomized design, in a 2 x 2 factorial arrangement. The factors were represented by two cottonseed processing method (whole or ground) and