Petersson Huff (ricerock53)

Atrazine (ATR), a bio accumulative herbicide is frequently used in agriculture to control unwanted weeds. Due to continuous application, atrazine persists in the environment and causes deleterious impacts including neurotoxicity, hepatotoxicity, and gut microbiota disorders. Therefore, this study for the first time reports the variation in the gut microbiota, induction of process of apoptosis and autophagy in mice induced by ATR. Results indicated that TUNEL-positive hepatocytes suggestive of apoptosis were increased in livers of different experimental mice. Results on metabolic analysis in liver tissues indicated an overall change in seventy-six metabolites particularly Uridine 5'-diphosphate, Propenoylcarnitine and Chinenoside V resulting in generation of energy-related metabolic disorders and imbalance of oxidation/autoxidation status. Results on gut microbiome inquisition showed that ATR changed the richness and diversity of gut microbiota of mice and number of Firmicutes. Moreover, results also revealed that ATR induced apoptosis via disruption of apoptotic (Bax, Bcl2, and Casp3) and autophagy (LC3/Map1lc3a, Beclin 1/Becn1 and P62/Sqstm1) genes. Results of our experimental study confirmed that changes in gut microbiota play a significant role in process of gut immune regulation and inflammation via different metabolites. In conclusion, the findings of our study provide a new idea for the involvement of mechanisms of detoxification in liver and inquisition of gut microbiota plays crucial role in regulation of physiological activities through liver-gut axis to mitigate toxic effects in animals.Di(2-ethylhexyl) phthalate (DEHP) is a priority environmental pollutant with carcinogenic, teratogenic, and mutagenic toxicity. Because it is widely used and ubiquitous in water, it is urgent to use a non-toxic, fast, and non-temperature dependent photocatalyst for degradation. Herein, a Z-scheme heterojunction composite catalyst consisting of Bi2O3 and TiO2 with reduced graphene oxide (rGO) as a two-dimensional template was designed and characterized. Under simulated solar radiation, the catalyst doped with 4% rGO presented the best photocatalytic DEHP (10 mg L-1) degradation at pH = 6, reaching 89% conversion in 90 min, and the degradation rate was 2.05 times higher than unmodified materials. The successful preparation of the Z-scheme junction enhanced the utilization of visible light region, thereby improving the DEHP's photocatalytic degradation performance. Subsequently, density functional theory (DFT) combined with GC-MS metabolite detection to propose a complete DEHP photocatalytic degradation mechanism. ·O2- and ·OH were detected as the primary reactive oxygen radicals involved in DEHP degradation, which easily attacked the O11 site with a high Fukui index (f0) through de-esterification, β-oxidation, and hydroxylation. While satisfying the rapid degradation, the highly repeatable catalyst cleaved the aromatic ring so that DEHP achieved mineralization during the degradation process. Therefore, its ability to completely degrade was very promising for environmental remediation, especially in water treatment. Besides, there were only a few studies on the degradation mechanism and reaction pathway of DEHP under visible light, which provided a theoretical basis for the aromatic compounds' photocatalysis research.A series of biodegradable copolyester of poly (butylene succinate-co-butylene malate) (P (BS-co-BM)) bearing hydroxyl groups were prepared by one-pot synthetic strategy without hydroxy-protection. The structure and properties of the P (BS-co-BM) were characterized by nuclear magnetic resonance (1H NMR), thermogravimetry analysis (TGA), differential scanning calorimetry (DSC), polarized optical microscope (POM), contact angle tester and enzymatic degradation. The results showed that the P (BS-co-BM) manifested excellent thermal properties. The glass transition temperature (Tg) of the P (BS-co-BM) increased with malic acid u