Neville Josefsen (pumabowl00)

We conclude by summarizing progress in current research, identifying areas for future exploration in drug development and nanotechnology, and discussing future prospects for management of this disease. Copyright ©2020, American Association for Cancer Research.The DNA-binding of two salphen-like metal complexes of nickel(II) (1) and zinc(II) (2) was investigated in different ionic strength solutions by absorption spectroscopy. The data analysis allowed us to obtain the values of their extrapolated DNA-binding constant in physiological conditions, with DNA-binding strength in the order Ni > Zn, and to give relative weight to the electrostatic and non-electrostatic contributions to their DNA-interaction. Understanding waste generation flow is vital to any evidence-based effort by policy-makers and practitioners to successfully manage construction project waste. Previous research has found that accumulative waste generation in construction projects follows an S-curve, but improving our understanding of waste generation requires its investigation at a higher level of granularity. Such efforts, however, are often constrained by lack of quality "bigger" data, i.e. data that is bigger than normal small data. This research aims to provide an anatomy of waste generation flow in building projects by making use of a large set of data on waste generation in 19 demolition, 59 foundation, and 54 new building projects undertaken in Hong Kong between 2011 and 2019. We know that waste is generated in far from a steady stream as it is always impacted by contingent factors. However, we do find that peaks of waste generation in foundation projects appear when project duration is at 50-85%, and in new building projects at 40-70% of total project time. Our research provides useful information for waste managers in developing their waste management plans, arranging waste hauling logistics, and benchmarking waste management performance. The global amount of soybean and wheat produced is about 350 and 750 million metric tons every year, respectively. In consequence, huge amounts of waste are produced from them. The aim of this work was to employ two wastes -soybean husk and flour mill waste- to produce high quantities of alpha-amylase enzyme. The substrate composition and the culture conditions were assayed to improve alpha-amylase production by solid-state fermentation employing the fungus Aspergillus oryzae. The maximum productivity of the enzyme was achieved using a culture substrate composed of the two wastes, at 45% soybean husk and 55% flour mill by-product, without pre-treatment, at an incubation temperature of 30 °C. The optimal incubation time (6 days), yielded a very high alpha-amylase activity (47,000 U/g dry substrate) at low-cost. The enzymatic extract obtained was characterized by LC-MS, providing a complete profile of the proteins produced during the solid-state fermentation on these two wastes. Then, the extract was purified in a single-step by size-exclusion chromatography and the recovery and the purification factor of alpha-amylase enzyme were about 83% and 6, respectively. The system was scaled up 50 times and yielded a similar enzymatic activity (45,900 U/g of dry substrate). A new type of biocatalyst was developed to facilitate the biochemical decomposition of 4-chlorophenol (4-CP) in this study. Oxydoreductases that catalyze the initial steps of 4-CP biodegradation were immobilized on a synthetic inorganic enzyme support. Type-X zeolite, a high-surface area support, was synthesized from coal fly ash, on which nickel ions were plated by impregnation (Ni-zeolite), followed by the effective immobilization (77.5% immobilization yield) of recombinant monooxygenase (CphC-I), dioxygenase (CphA-I), and flavin reductase (Fre) isolated from Pseudarthrobacter chlorophenolicus A6 and Escherichia coli K-12, respectively. The retained catalytic activity of the enzymes immobilized on Ni-zeolite was as high as