Hurley Roberts (pullorchid11)

The maintenance of deoxyribonucleotide triphosphate (dNTP) homeostasis through synthesis and degradation is critical for accurate genomic and mitochondrial DNA replication fidelity. Trypanosoma brucei makes use of both the salvage and de novo pathways for the provision of pyrimidine dNTPs. In this respect, the sterile α motif and histidine-aspartate domain-containing protein 1 (SAMHD1) appears to be the most relevant dNTPase controlling dNTP/deoxynucleoside homeostasis in mammalian cells. Here, we have characterized the role of a unique trypanosomal SAMHD1 orthologue denominated TbHD52. Our results show that TbHD52 is a mitochondrial enzyme essential in bloodstream forms of T. brucei. Knockout cells are pyrimidine auxotrophs that exhibit strong defects in genomic integrity, cell cycle progression, and nuclear DNA and kinetoplast segregation in the absence of extracellular thymidine. The lack of TbHD52 can be counteracted by the overexpression of human dCMP deaminase, an enzyme that is directly involved in dUMP formation yet absent in trypanosomes. Furthermore, the cellular dNTP quantification and metabolomic analysis of TbHD52 null mutants revealed perturbations in the nucleotide metabolism with a substantial accumulation of dCTP and cytosine-derived metabolites while dTTP formation was significantly reduced. We propose that this HD-domain-containing protein unique to kinetoplastids plays an essential role in pyrimidine dNTP homeostasis and contributes to the provision of deoxycytidine required for cellular dTTP biosynthesis. Fractures of the distal humerus represent 70% of fractures in children under 12 years. It is necessary to have knowledge about this type of injury, the therapeutic alternatives, and the possible complications. To assess the functional results of the AO fixation technique in the management of humeral supracondylar fractures in pediatric patients. Patients admitted to the pediatric orthopedics service were surgically treated by external fixation AO method, from July 1 to December 31, 2018. The data were obtained by monitoring from admission of patients until discharge from the outpatient clinic performing the functional clinical evaluation using the Mayo Elbow Performance Score to evaluate the function of the elbow. We included 5 patients with humeral supracondylar fracture treated by external fixation AO method, with an average age of 6 years. Of the 5 cases, 3 (60%) suffered the right upper limb injury and 2 (40%) the upper left limb, 1 patient (20%) had partial radial nerve injury. The score obtained on the Mayo Elbow Performance Score was 80 points on average. The functional result of the treatment of humeral supracondylar fractures by means of AO external fixation technique, shows good results, positioning it as an alternative therapeutic treatment option. The functional result of the treatment of humeral supracondylar fractures by means of AO external fixation technique, shows good results, positioning it as an alternative therapeutic treatment option. The floating shoulder has an incidence of 0.1% of all fractures, the result of high-energy trauma. There is still no consensus in the literature on the best treatment surgical versus conservative. Patients with floating shoulder diagnosis were evaluated from January 2013 to December 2018, collecting initial data from the electronic file. The morphological types of fractures were described using the Allman and AO classifications. Patients were evaluated using the UCLA scale for shoulder functionality. From 14 patients, 4 were excluded due to lack of follow-up. Of the remaining 10, 9 were men, 1 woman. JDQ443 The average age was 29 years (range 15-42 years). Three patients had left injury, and 7 patients, right; 6 patients hurt the dominant limb. The most common morphological pattern was glenoid neck fracture + clavicle fracture in 7 patients. Surgical treatment w