Freeman Abel (porchbutter5)

Effective delivery system for oral insulin administration is a promising way for diabetes therapy. Herein, we prepared alginate microbeads containing chitosan nanoparticles (CNP) for controlled release of insulin. CNP was developed by reaction between tripolyphosphate (TPP) and chitosan. The ratio of TPP to chitosan was optimized aiming with smaller and more unified distributed CNP. TEM and DLS analysis confirmed that CNP has size around 150 nm with low PDI value and strong surface charge. Encapsulate ability for bovine serum albumin, working as model protein, was 11.45%, and the encapsulate efficiency was 23.70%. To modify the release profile of protein suitable for oral insulin delivery, sodium alginate was applied to coat on the surface of CNP by electrostatic interaction. After that, CaCl2 was added to reinforce the alginate coating layer. FTIR analysis confirmed the interaction of alginate with chitosan and reaction with calcium ion. After reaction with Ca2+ ion, size measurement revealed that CNP was incorporated into alginate microbeads with mean diameter about 3.197 μm. Alginate microbeads presented irregular shape with small particles inside as revealed by optical microscope. Meanwhile, the release test demonstrated that protein release was pH-dependent. Acidic pH value retards protein release and neutral pH value promotes protein release. At last, insulin-loaded alginate microbeads were administrated to hyperglycemia model mice and blood glucose profile was monitored afterward. Insulin-loaded microbeads significantly lowered blood glucose level compared with mice treated with alginate microbeads without insulin. CC930 It is noted that insulin-loaded alginate microbeads could lower blood glucose level in much prolonged period of 96 h, indicating that insulin was released in controlled manner.Ethanol was produced by separate hydrolysis and fermentation using Azolla filiculoides as a biomass. Thermal acid hydrolysis and enzymatic saccharification were used as pretreatment methods to produce monosaccharides from Azolla. The optimal content for thermal acid hydrolysis of 14% (w/v) Azolla weed slurry produced 16.7-g/L monosaccharides by using 200 mM H2SO4 at 121 °C for 60 min. Enzymatic saccharification using 16 U/mL Viscozyme produced 61.6 g/L monosaccharide at 48 h. Ethanol productions with ethanol yield coefficients from Azolla weed hydrolysate using Kluyveromyces marxianus, Candida lusitaniae Saccharomyces cerevisiae, and Pichia stipitis were 26.8 g/L (YEtOH = 0.43), 23.2 g/L (YEtOH = 0.37), 18.2 g/L (YEtOH = 0.29), and 13.7 g/L (YEtOH = 0.22), respectively. Saccharomyces cerevisiae produces the lowest yield as it utilized only glucose. Bioethanol from Azolla weed hydrolysate can be successfully produced by using Kluyveromyces marxianus because it consumed the mixture of glucose and xylose completely within 60 h.The surgical treatment contributes to broad variety of cardiovascular diseases (CVD). Due to many involved factors in preoperative bleeding, it is almost difficult to perform better Haemostatic approach. Fibrinogen is a major blood glycoprotein and a coagulation factor which decreases postoperative bleeding. It has a potential role in platelet activation and bleeding inhibition; it may reflect the inflammatory responses and be related to the endothelial dysfunction. Fibrinogen can act as a pro-inflammatory element via increasing some inflammatory markers including IL-6, tumor necrosis factor-α (TNF-α), monocyte chemo attractant protein (MCP-1), macrophage inflammatory protein-1 (MIP-1a and b), matrix metalloproteinase (MMP-1 and MMP-9) and Toll-like Receptors (TLRs); through activation of these factors, fibrinogen may induce some inflammatory mechanisms such as focal adhesion kinase (FAK), mitogen-activated protein kinases (MAPK) and nuclear factor κB (NF-κB) pathways. It may cause endothelial dysfunction by increasing P and E-selection, intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion