Simpson Baird (pikebeat6)

The selective laser melting of Ti6Al4V would induce definite changes in the microstructure that may affect its corrosion properties. Microstructural examination showed the formation of relatively thin beta (β) lamella in selective laser melted (SLM) Ti6Al4V compared to wrought Ti6Al4V. X-ray diffraction analysis (XRD) analysis confirmed the presence of alpha and beta phases in both SLM and wrought Ti6Al4V. However, the higher concentration of the β phase in SLM Ti6Al4V compared to wrought Ti6Al4V was evident in the microstructure. As candidate dental implant materials, the corrosion behavior of both SLM and wrought Ti6Al4V was assessed in artificial saliva (AS) and deionized water (DI) containing various species i.e. fluoride (F), calcium chloride (CaCl2) and lactic acid (LA). Electrochemical impedance spectroscopy and potentiodynamic polarization analysis was carried out to estimate the corrosion behavior of SLM and wrought Ti6Al4V at room temperature. SLM Ti6Al4V offered better corrosion resistance than wrought Ti6Al4V in all solutions at pH > 6. However, wrought Ti6Al4V comparatively presented high corrosion resistance in AS + LA, DI + CaCl2 and DI + LA solutions (pH 6) was attributed to larger β content in the microstructure compared to wrought Ti6Al4V.This work aimed to the development of chitosan and protein isolate composite hydrogels, for carotenoids-controlled delivery and wound healing. 7,12-Dimethylbenz[a]anthracene mouse By increasing the concentration of the protein isolate, chitosan hydrogels were more elastic at a protein isolate concentration not exceeding 15% (w/w). Chitosan-protein isolate composite hydrogels revealed low cytotoxicity towards MG-63 osteosarcoma cells. Thanks to its appropriate structural, swelling and mechanical resistance properties, chitosan hydrogel (3%; w/v), reinforced with 15% (w/w) of protein isolate, was selected for the carotenoids in vitro release study. Release profiles, show delivery patterns, where carotenoids were more barely released at a pH 7.4 medium (p less then .05), compared to more acidic microenvironments (pH 4.0 and pH 2.0). Thus, developed hydrogels could be applied as pH-sensitive intelligent carriers, for drugs-controlled release, with interesting antioxidant abilities. The in vivo healing potential of hydrogels in rats' models was further studied. Topical application of hydrogel-based patches allowed the acceleration of wound healing and the complete healing, for composite hydrogel enriched with carotenoids.Hydrophobin-1 (HFB-1) found on the surface of fungal spores, plays a role in the lack of antigen recognition by the host immune system. The present study aimed to evaluate the potential application of HFB-1 for the delivery of doxorubicin (Dox) into different cell lines. Coating the surface of niosomes (Nio) with HFB-1 leads to the hypothesis that this protein can confer protection against in vivo immune-system recognition and prevent the immune response. Thus, HFB-1 could become a promising alternative to polyethylene glycol (PEG). Here, HFB-1-coated niosome loaded with doxorubicin (Dox) based on Span 40, Tween 40 and cholesterol was prepared and compared with the PEG-coated niosome. Physicochemical characteristics of the prepared formulations in terms of size, zeta potential, polydispersity index (PDI), morphology, entrapment efficiency (EE), and release rate were evaluated at different pH levels (2, 5.2, and 7.4). In the end, the in vitro cytotoxicity assay was performed on four different cancer cell lines namely A549, MDA-MB-231, C6 and PC12 in addition to one control cell line (3 T3) to ensure the formulation's selectivity against cancer cells. Results showed that the niosomes coated with HFB-1 presented better size distribution, higher EE, more sustained release profile, enhanced biocompatibility and improved anticancer effects as compared to the PEG-coated niosomes. Interestingly, the viability percentage of the control cell line was higher than dif