Allison Stark (olivekale5)

Loricrin is usually observed in abundance in keratinizing epithelium in response to mechanical stress, which may be associated with development and malignant transformations in conditions such as oral submucous fibrosis (OSMF). Therefore, understanding of various molecular mechanisms associated with difference in gene expressions between OSMF and that of normal oral tissue is important. The aim of this study was to evaluate of the efficacy of loricrin as a diagnostic marker in patients with OSMF. Fifty formalin-fixed paraffin-embedded tissue blocks were obtained from the archives of the department. The study sample was grouped into two groups of normal mucosa (group I; = 20) and OSMF (group II; = 30) specimens. The study tissues were immunohistochemically stained with loricrin antibody and were further graded on basis of staining intensity. Loricrin immunostaining was observed significantly more in OSMF cases and even in stratum granulosum in comparison to normal mucosa. Loricrin can act as an early indicator and a prognostic marker for detection of deleterious changes within epithelium in OSMF. Loricrin can act as an early indicator and a prognostic marker for detection of deleterious changes within epithelium in OSMF.The study aimed to compare the extent of dentinal microcracks in the radicular dentin using hand K files and various types of rotary nickel-titanium (NiTi) file systems. Forty mandibular incisor teeth (human) were grouped for the study. The root apices were closed, and the canals were straight ( less then 5°). The samples were divided into four groups (n = 10). Group 1-Hand K file system (SybronEndo, SA), Group 2-XP-endo Shaper (FKG Dentaire, La Chaux-de-Fonds, Switzerland), Groups 3-Neoendoflex file (Neoendo, Orikam, India), and Group 4-Hero Shaper files (Micro-Mega, Becacon, France). Sectioning was done at 3, 6, and 9 mm from the apex, perpendicular to the long axis using a diamond disc under water coolant. The cut samples were then observed through a scanning electron microscope at ×50 enlargement. The control group showed no microcracks except one sample in the coronal third, which was less significant. The difference in the amount of crack detected on comparing the different groups was significant. In the experimental groups, XP-endo Shaper group showed significantly less number of cracks than the Neoendo flex, and Hero Shaper groups. learn more Within the limitations of this study, all the system, except hand K files, produced remarkable dentinal microcracks. Least cracks were seen in canals instrumented with XP-endo Shaper. It can also be noted that single file system causes less number of microcracks. To achieve effective infection control only disinfecting instruments is not perfect when sterilization is an ideal method. Few chemical disinfection methods have disadvantage of not killing spores as cross infection is of great importance in dentistry; Standard sterilization and disinfection protocols must be followed by dental health care professionals for efficient infection control. The aim of this study was to evaluate the efficacy of undiluted concentrations of Durr Dental system, Bacillol, Savlon, and Dettol for disinfection of striated and nonstriated orthodontic instruments. Orthodontic instruments were divided into two groups. Each group of instrument was exposed to three microbes , , and . Once the instruments were exposed to bacterium, they were immersed in four commercially available disinfectants Durr Dental solution, Bacillol, Dettol, and Savlon. Culture streaks were taken at 5, 10, and 15 min of contact time and growth of organisms was observed on culture media. All the four disinfectants showed no growth of bacteria and all were significantly effective. As per the immersion time factor, Durr system and Bacillol were more efficient than Dettol and Savlon.