Holder Whittaker (lacefrog1)

Circular RNAs (circRNAs) are a type of noncoding RNA (ncRNA) and have attracted widespread research attention in recent years. They form a covalently closed loop structure and are widely distributed in tissues and organs. CircRNAs have also been found to regulate a variety of biological processes by functioning as microRNA sponges, transcriptional regulators, and protein binding partners. Recent sequencing results have shown that specific circRNA expression patterns are significantly associated with adult stem cell self-renewal and differentiation, indicating that the differential expression of circRNA affects whether adult stem cells initiate the differentiation process. By studying circRNA expression during adult stem cell differentiation into different lineages, such as the osteogenic, chondrogenic, adipogenic, neurogenic, and cardiac lineages, it can be understood that circRNAs are a potential factor regulating adult stem cell differentiation. Herein, we summarize the general characteristics and functional mechanisms of circRNAs and further examine their roles in adult stem cell self-renewal and differentiation. STUDY DESIGN Case series. BACKGROUND AFOs are a commonly prescribed medical device given to children with cerebral palsy (CP) in an attempt to improve their gait. The current literature is equivocal on the effects AFOs have on the gait of children with CP. The vast majority of AFOs issued are not subject to AFO-FC tuning. There are emerging studies investigating the effects tuning AFO-FCs has on the gait of children with CP. However, the research is limited, and there is a lack of quantitative data. OBJECTIVE To compare the kinematics of tuned versus non-tuned gait in children with CP. METHODS Gait analysis assessment of five children aged between 7-11 years with a diagnosis of CP (one hemiplegic and four diplegic participants, two female, three male, with a Gross Motor Functional Classification System (GMFCS) of 2) at a Gait Analysis Laboratory. RESULTS In comparison to barefoot and non-tuned gait, walking with a tuned AFO-FC produced improvements in several key gait parameters. Including hip flexion and extension, posterior pelvic tilt and knee extension. Results also indicated that the type of gait pattern demonstrated by the participant affected the outcomes of tuning. CONCLUSIONS Tuning the AFO-FC of children with CP has the potential to improve hip function, pelvic function, knee extension in stance phase and knee flexion during swing phase and that a non-tuned AFO-FC can potentially decrease hip function, posterior pelvic tilt and increase knee extension. CLINICAL RELEVANCE Whilst AFO-FC tuning has been recommended for routine clinical practice, there still remains a paucity of research on the kinematic effects of using a tuned AFO-FC compared to a non-tuned. This paper provides a comparison of kinematics on children with CP, during barefoot, non-tuned and tuned AFO-FC walking with a view to inform clinical practice. The aim of the study was to determine the differences in the microbiological, physical-chemical, aromatic and phenolic composition between two winemaking methods carbonic maceration and the standard method of destemming and crushing. We analysed 84 commercial Rioja wines made from the Tempranillo grape variety during the 2017 vintage, 40 had been made by carbonic maceration and 44 by destemming and crushing. Despite the heterogeneity within the two groups of wines, it was possible to differentiate between them. Wines made by carbonic maceration presented higher aromatic quality due to their higher total content of esters and acetates, as well as a greater colour intensity due to a higher phenolic content and higher rates of ionization and polymerization. In addition, it was observed that the antioxidant activity, the content in coumaroyl derivatives of anthocyanins and the vitisins A and B were considerably greater in wines made by carbonic maceration. A rapid and sensitive immunochromatograph