Henson Vargas (kenyaisland81)

In this work is presented the development of a method for As and Se determination in crude palm oil samples by hydride generation atomic fluorescence spectrometry and Hg by cold vapor atomic fluorescence spectrometry after ultrasound-assisted emulsification and extraction induced by emulsion breaking (EIEB). The optimization of the method was carried out by multivariate designs. The developed method has presented limits of quantification (LOQ) of 0.72, 0.12, and 1.5 μg L-1 for As, Hg, and Se, respectively. The precisions of the proposed method expressed as repeatability were 0.92, 2.2, and 3.7% RSD for 2 µg L-1 (n = 10) of As, Hg and Se, respectively. The developed methodology was applied in palm oil samples collected in the Bahia State. Concentrations (μg L-1) found in the samples were between less then LOQ - 1.3 for As and 3.0-15 for Se. For Hg, all analyzed samples were below the limit of quantification. In this study, the occurrence of cannabinoids in hemp-based food products was investigated. For that purpose, a new liquid chromatography tandem mass spectrometry method for the quantification of fifteen cannabinoids was developed and validated for multiple matrices. Method performances were good, fulfilling the SANTE/11813/2017 requirements, and allowing for products compliance testing with various national legislations on cannabinoids levels in food products. The limit of quantification of each analyte was 0.15 mg/kg for hemp seed and hemp protein, 0.6 mg/kg for hemp seed oil, and 0.005 mg/kg for raw milk and milk powder. The applicability of the method was further demonstrated by conducting a limited survey on twenty hemp-based food products. The survey revealed that products from the same category can have very different cannabinoids profiles and levels. These results highlighted the importance of cannabinoids testing of food products in view of the current heterogeneous and fast evolving regulatory landscape worldwide. A sensor consisting of an optical fibre with the exposed tip coated with the polyoxometalate salt [(C4H9)4N]4H[PMo10V2O40], specially designed to be insoluble in water, which UV-Vis spectrum changed in contact with formaldehyde, is presented. The sensor limit of detection for formaldehyde was 0.2 mg L-1, and the limit of quantification was 0.6 mg L-1, which were close to the conventional spectrophotometric method values of 0.2 mg L-1 and 0.5 mg L-1, respectively, and lower than the tolerable limit for ingested food. The sensor was tested for formaldehyde quantification in milk, as its deliberate addition is a matter of concern. The results obtained analysing formaldehyde in milk samples by the optical sensor and by the conventional method were not statistically different (α = 0.05). Colorful fruits conveying astringent beverages providing well documented health-benefits may present clarification, turbidity and sedimentation problems. Hydraulic pressing whole 'Wonderful' pomegranates and ultrafiltration followed by high temperature short-time pasteurization was used to mimic the dominant U.S. commercial juicing protocol, delivering not-from-concentrate juices, stored at 4 and 25 °C for 3 months. A goal was to avoid expensive and complicated clarification and fining steps. Data were subjected to an analysis of variance and principal components analysis. Changes in quality attributes, organic acids and anthocyanidins are presented. Hydraulic pressing, ultrafiltration and initial pasteurization had minor effects on color parameters, organic acids and anthocyanidins. There were no significant temperature, time or factorial effects for all color parameters during storage of the not-from-concentrate pasteurized juices. However, there were significant time- and temperature-effect decreases in organic acids and anthocyanidins. This is the first report on quality attributes in ultrafiltered, pasteurized