Goldman Astrup (inchman3)
The influence of commercial prebiotics (fructo-oligosaccharides and inulin) and sugars (glucose and sucrose) on enhancing equol production from soymilk isoflavones by Bifidobacterium longum BB536 and Bifidobacterium breve ATCC 15700 was evaluated in vitro. Sterilized soymilk was inoculated with each bacterial species at 37 °C for 48 h. The growth and β-glucosidase enzyme activity for the two Bifidobacterium species in soymilk throughout fermentation were assessed. The highest viable count for B. breve (8.75 log CFU/ml) was reached at 36 h and for B. longum (8.55 log CFU/ml) at 24 h. Both bacterial species displayed β-glucosidase activity. B. breve showed increased enzyme activity (4.126 U) at 36 h, while B. longum exhibited maximum activity (3.935 U) at 24 h of fermentation. Among the prebiotics screened for their effect in isoflavones transformation to equol, inulin delivered the highest effect on equol production. The co-culture of B. longum BB536 and B. breve ATCC15700 in soymilk supplemented with inulin produced the highest level (11.49 mmol/l) of equol at 48 h of fermentation process. Level of daidzin declined whereas that of daidzein increased, and then gradually decreased due to formation of equol when soymilk was fermented using bifidobacterial. This suggests that the nutritional value of soymilk may be increased by increasing bioavailability of the bioactive ingredients. Collectively these data identify probiotics and prebiotic combinations suitable for inclusion in soymilk to enhance equol production.Air pollution due to haphazard industrialization has become a major concern in developing countries. Yet, enforcement of related norms remains problematic because violators cannot easily be pinpointed among closely situated industrial units. Accordingly, it has become imperative to equip regulatory authorities with an economical yet accurate tool that quickly locates emission sources and estimates emission rates. Against this backdrop, we propose RESILIENT, a method for Robust Estimation of Source Information from LImited field measuremENTs, which exhibits significant statistical robustness and accuracy even when the data are collected using a low-cost error-prone sensor. In our field experiment, where ground truth was unavailable, the sources estimated to be inactive based on the complete set of measurements were found inactive (up to three decimal places of accuracy) at least 72% of the time even when estimated using just 54% of random measurements. In that setting, rate estimates of active sources were also found to be statistically robust. For direct validation of RESILIENT, we considered a separate public dataset involving 10 tracer experiments, and obtained a significant correlation coefficient of 0.89 between estimated and recorded emission rates, and that of 0.99 between predicted and measured concentration levels at sensor locations.Mycotoxins present a great concern to food safety and security due to their adverse health and socio-economic impacts. The necessity to formulate novel strategies that can mitigate the economic and health effects associated with mycotoxin contamination of food and feed commodities without any impact on public health, quality and nutritional value of food and feed, economy and trade industry become imperative. Various strategies have been adopted to mitigate mycotoxin contamination but often fall short of the required efficacy. One of the promising approaches is the use of bioactive plant components/metabolites synergistically with mycotoxin-absorbing components in order to limit exposure to these toxins and associated negative health effects. In particular, is the fabrication of β-cyclodextrin-based nanosponges encapsulated with bioactive compounds of plant origin to inhibit toxigenic fungi and decontaminate mycotoxins in food and feed without leaving any health and environmental hazard to the consumers. The present paper reviews the use of botanicals extracts and their phytochemic