Jeppesen Munro (foldhedge21)

The discovery of large transcripts of long RNAs that have limited protein coding capacity, known as long non-coding RNAs (lncRNAs) present new concepts on RNA-mediated gene regulation. Increasing evidence suggests that large intervening ncRNAs regulate key pathways in cancer genesis and metastasis. Among the most characterized lncRNAs, homeobox (HOX) transcript antisense intergenic RNA (HOTAIR) acts as an oncogenic molecule in different cancer cells, and thus its expression level serves as a potential biomarker for diagnostic and therapeutic purposes in several human cancers, such as breast, prostate, liver and ovarian cancer. This paper reports a simple and sensitive sensor platform for the detection of HOTAIR. Extracted HOTAIR sequences from ovarian cancer cells and plasma samples derived from ovarian cancer patients were magnetically isolated and purified, followed by a sandwich hybridization event at a screen-printed gold electrode. This event was monitored by amperometry using the hydrogen peroxide/horseradish peroxidase/hydroquinone (H2O2/HRP/HQ) system. The catalytic enhancement of the amperometric signal enabled our assay to achieve a detection limit of 1.0 fM with a good inter-assay reproducibility (relative standard deviation (%RSD) = less then 5.0%, n = 3). The method was used for the analysis of specific HOTAIR in cell line and a small cohort of plasma samples derived from patients with ovarian cancer. The analytical performance of the method was also demonstrated using a standard RT-qPCR. We believe that the proof of the concept assay demonstrated here could be a cost-effective alternative platform for screening cancer-related lncRNAs in routine clinical settings.Electrochemiluminescence resonance energy transfer (ECL-RET) assay as an efficient analytical technique has aroused considerable interest in recognition and biosensing. In the present study, a novel self-enhanced ECL-RET of Ru(bpy)2(phen-NH2)2+ as an efficient luminophore to the MoS2 nanosheets as effective quencher was designed for CA19-9 antigen analysis. Herein, the graphene oxide grafted hyperbranched aromatic polyamide (GO-HBP) with high loading ability for Ru-complex was used as the sensing platform, while amine-rich nitrogen-doped carbon nanodots (NCNDs) which covalently linked to Ru(bpy)2(phen-NH2)2+ applied as co-reactant for enhancing of anodic ECL signal response. In this approach GO-HBP-Ru-complex-NCND-anti-CA19-9 Ab exhibited amplified ECL emissions ("on" state) and with formation of sandwiched immunocomplex between, immobilized CA19-9 Ab, CA19-9 antigen and MoS2 nanosheets modified with CA19 9 antibody, the ECL response of luminophore was efficiently quenched ("off" state). The signal response was doubly amplified by covalent attachment of more luminophore, co-reactant and CA19-9 antibody with hyperbranched aromatic polyamide. On the basis of all above features, the ECL intensity of Ru-NCND decreased with the increase of the concentration of CA19-9 antigen in a wide linear range of 2 mU mL-1- 50 U mL-1 with the detection limit of 0.25 mU mL-1 (S/N = 3). The application of the fabricated ECL-RET immunosensor for determination of CA19-9 antigen in human serum samples was appraised that the satisfactory results were found to be in admissible accord with those gained with the reference method (ELISA assay).The suggested method opens up a novel avenue for expanding high-performance ECL-RET immunosensors cancer markers detection in clinical monitoring.High working temperature is the main obstacle in the design of chemiluminescence gas sensor. In this paper, a novel formaldehyde gas sensor based on chemiluminescence on nano-Pt/Mo4V6Ti10O47 at lower temperature than 200 °C was reported. Formaldehyde can be oxidized on the catalyst and emit chemiluminescence of specific wavelength. The relative standard deviation (RSD) of the chemiluminescence intensities within 600 h by continually introducing 20 mg/m3 formaldehyde is less than 3%. There is a good