Kamp Steen (flutebill87)

Extracellular vesicle (EV)-mimetic nanovesicles (NVs) from embryonic stem cells have been observed to stimulate neurovascular regeneration in the streptozotocin-induced diabetic mouse. Pericytes play important roles in maintaining penile erection, yet no previous studies have explored the effects of pericyte-derived NVs (PC-NVs) in neurovascular regeneration in the context of erectile dysfunction. To investigate the potential effect of PC-NVs in neurovascular regeneration. PC-NVs were isolated from mouse cavernous pericytes, and neurovascular regeneration was evaluated in an invitro study. Twelve-week-old C57BL/6J mice were used to prepare cavernous nerve injury model. Erectile function evaluation, histologic examination of the penis, and Western blots were assessed 2weeks after model creation and PC-NVs treatment. The main outcomes of this study are PC-NVs characterization, intracavernous pressure, neurovascular regeneration in the penis, and invitro functional evaluation. The PC-NVs were extractedation in a Mouse Model of Cavernous Nerve Injury. J Sex Med 2020;172118-2128.The objectives of the study was to determine the types, challenges and implications of surveillance methods for controlling Covid-19 pandemic. An integrative article review was done. The source of data were documents from WHO, Euro-surveillance, CDC, Saudi CDC, MOH, and journals from PubMed, Medline, etc. The inclusion searching criteria were surveillance, Covid-19, types, benefits and challenges, during the period 2005-2020. Published studies, reviews and guidelines that determined these criteria were collected. Data extraction and analysis were completed for all included articles. A critical appraisal was done based on the University of Michigan Practice Guideline's levels of evidence. The final sample for the integrative review comprised 30 studies. Results revealed that types of Covid-9 surveillance includes routine surveillance (comprehensive, case-based, and aggregated weakly methods), active, wildlife, syndromic, sentinel and sentinel-syndromic methods. Laboratory and hospital-based surveillance are another important types. Help-lines, surveys, participatory electronic, digital and event-based surveillance are relatively new cost-effective methods. Many surveillance indicators can be calculated. Timely and accurate of surveillance data is an essential element for effective Covid-19 interventions. Regarding challenges, the quality of surveillance in developing countries is constrained by resources and training. The main limitations of surveillance are under-ascertainment/under-reporting, lack of timeliness and completeness of surveillance data. In conclusion, surveillance is a cornerstones for controlling Covid-19 pandemic. Enhancing Covid-19 surveillance is vital for rapid cases detection, containing spread & ending pandemic. The interaction of specially designed nanoparticles with proteins is the basis of formation of a nanoparticle-protein corona. Silver nanoparticles and molecules have been used in many fields due to their strong antimicrobial activity against pathogenic microorganisms such as bacteria, yeast and fungi. E. coli is a Gram-negative bacteria that has the genome completely sequenced and determined majority of its protein 3D structures. Nitrate Reductase A is a cellular protein often found in many bacteria that uses nitrate as an electron acceptor during anaerobic growth. The enzyme is composed of three different chains α, β and γ, all having properties of metal binding regions and domains. Bioinformatics tools were used to investigate the structure, domains, interactomes, and docking sites of E. coli Nitrate Reductase A in order to predict the possible site of interaction of silver nitrate AgNO with the protein. The 3D structure of the NAR A protein was predicted with the Phyre2 protein modeling software. Thecid sequences were identified; 3D structure of sub