Blake Powers (flightbubble8)

Specific inhibitor of PTP4A3 significantly inhibited 20 μM endosulfan-induced cell migration, the expression and phosphorylation of Src and phosphorylation of focal adhesion kinase (FAK). Exposure to endosulfan resulted in activation of various signaling pathways including phosphoinositide 3-kinase (PI3K)/AKT, mitogen-activated protein kinase (MAPK) and nuclear factor kappa B (NF-κB), which were suppressed by PTP4A3 inhibitor or specific inhibitor for each signaling pathway. Exposure to endosulfan significantly reduced nitric oxide production and caused oxidative stress in HUVECs. These findings suggest that endosulfan promoted cell migration through PTP4A3-mediated various signaling pathways in endothelial cells. Fractions, bioavailability, health risks of fine particulate maters (PM2.5)-bound potentially toxic elements (PTEs) (Pb, Cd, Cr, Cu and Zn) were investigated before and after coal limiting in Baoding city. The winter PM2.5 samples were collected at different functional areas such as residential area (RA), industrial area (IA), suburb (SB), street (ST) and Botanical Garden Park (BG) in 2016 (coal dominated year) and 2017 (gas dominated year). The fractions and bioavailability of PTEs were determined and evaluated based on BCR sequential extraction. Health risks through inhalation exposure were evaluated by US EPA health risk assessment model. The results from different years and functional areas were compared and discussed. The fractions and bioavailability of PM2.5-bound PTEs varied with functional areas. The percentages of cadmium (Cd) and zinc (Zn) in acid-soluble fraction (F1-Cd and F1-Zn) to the total amount of Cd and Zn were low in BG samples (p  less then  0.05). Bioavailability of Cd were high in SB samples (p  less then  0.05). Total contents of PM-bound PTEs in 2017 generally decreased compared with 2016. The differences of fraction and bioavailability between 2016 and 2017 depended on the elements and areas. Higher proportions of copper (Cu) in acid-soluble fraction (F1-Cu) and bioavailability of Cu (p  less then  0.05) were found in 2017 samples. Significant differences were found just at IA and RA for Pb, Cd and Zn. Our results indicated that the health risks from inhalation exposure for PTEs in PM2.5 declined about 11%-52% after the coal limiting in this city. Oysters accumulate high concentrations of zinc (Zn) and copper (Cu), which can be transferred to human due to sea food consumption. Breeding new oyster varieties with low Zn and Cu accumulations is one important way to improve food safety. However, the genetic basis for metal accumulation in mollusks is not well understood. To address this issue, oysters collected in the field were used for genome-wide association study (GWAS) and then the identified genes were used for mRNA expressions analysis in laboratory. First, GWAS were conducted for Zn and Cu accumulation in 288 wild Pacific oysters (Crassostrea gigas) farmed in the same ocean environment. The oysters did not show obvious population structure or kinship but exhibited 8.43- and 10.0- fold changes of Zn and Cu contents respectively. EPZ5676 GWAS have identified 11 and 12 single nucleotide polymorphisms (SNPs) associated with Zn and Cu, respectively, as well as 16 genes, which were Zn-containing proteins or participated in caveolae-dependent endocytosis. Second, the mRNA expressions of these 16 genes were observed under Zn and Cu exposure. After 9 days of Zn exposure, Zn contents increased 3.1-fold, while the mRNA expression of cell number regulator 3 increased 1.65-fold. Under 9 days of Cu exposure, Cu contents increased 1.97-fold, while the mRNA expression of caveolin-1 decreased 0.61-fold. These provide the evidence for their roles in regulating physiological levels of these two metals. The findings advance our understanding of the genetic basis of Zn and Cu accumulation in mollusks, which can be useful for breeding new, less toxic varieties of oysters