McConnell Gardner (doubtwoolen20)

Nutrient source and transport study in tropical creeks adjacent to megacities are sparse on a regional and global scale. High-frequency chemical data collected during 2017-2018 in the Thane creek system, the largest micro-tidal urbanized creek in Asia, were analysed to assess the chemical water quality, with its linkage to different hydrological stages (southwest monsoon, post- and pre-monsoon) and ongoing anthropogenic activities. Cluster analysis indicates typical zonation between creek outflow and nearshore waters with distinctive physicochemical properties. The creek outflow upholds substantial amounts of nutrient and suspended sediment due to turbid water movement from the ephemeral mudflats and anthropogenic dredging. The year-round hyper-turbid condition in the bottom water triggers the addition of disproportionate nutrients (9% dissolved inorganic nitrogen (DIN) and 14% reactive phosphorous (PO43-) in the outflow region. The DIN and PO43- removal up to 10 and 35%, respectively, occurs in the nearshore region; sedimentation, which acts as a sinking interface for nitrogen and phosphorous, also causes shifting in their limiting conditions. The hyper-turbid condition causes removal of dissolved silicate (DSi) by 5% in the entire region. Ammonium (NH4+) is mainly sourced from the sewage in outflow waters and efficiently mineralized. Chemical indexing of water highlights that the bottom water column remained un-supportive to the balanced ecology. The findings of this study have implications for other tropical creek-estuary concerning management strategies against inadequate flushing. Gusacitinib mouse The stalled nutrient export affected balance ecology in tropical Thane creek, which need attention in order to accurately understand the impact on adjacent marine environment and to form effective mitigation policies.Dravet syndrome (DS) is a rare and severe epileptic syndrome of childhood with prevalence between 1/22,000 and 1/49,900 of live births. Approximately 80% of patients with this syndrome present SCN1A pathogenic variants, which encodes an alpha subunit of a neural voltage-dependent sodium channel. There is a correlation between PCDH19 pathogenic variants, encodes the protocadherin 19, and a similar disease to DS known as DS-like phenotype. The present review aims to clarify the differences between DS and DS-like phenotype according to the SCN1A and PCDH19 variants. A systematic review was conducted in PubMed and Virtual Health Library (VHL) databases, using "Dravet Syndrome" and "Severe Myoclonic Epilepsy in Infancy (SMEI)" search words, selecting cohort of studies published in journal with impact factor of two or greater. The systematic review was according to the Preferred Reporting Items for Systematic Review and Meta-Analysis recommendations. Nineteen studies were included in the present review, and a significant proportion of patients with DS-carrying SCN1A was greater than patients with DS-like phenotype-harboring PCDH19 variants (76.6% versus 23.4%). When clinical and genetic data were correlated, autism was predominantly observed in patients with DS-like-carrying PCDH19 variants compared to SCN1A variant carriers (62.5% versus 37.5%, respectively, P-value = 0.044, P-value corrected = 0.198). In addition, it was noticed a significant predisposition to hyperthermia during epilepsy crisis in individuals carrying PCDH19 variants (P-value = 0.003; P-value corrected = 0.027). The present review is the first to point out differences between the DS and DS-like phenotype according to the SCN1A and PCDH19 variants. Development of a system for direct lactose to ethanol fermentation provides a market for the massive amounts of underutilized whey permeate made by the dairy industry. For this system, glucose and galactose metabolism were uncoupled in Saccharomyces cerevisiae by deleting two negative regulatory genes, GAL80 and MIG1, and introducing the essential lactose hydrolase LAC4 and lactose transporter LAC1