Holt Hurley (cubtime65)

Therefore, NSL 12 is selected for toxicological studies and was found safe in both, in vitro and in vivo studies.The aim of this study was to improve the physicochemical stability of neohesperidin (NH) using nanoliposomal encapsulation in association with surface decoration strategy employing chitosan (CH) and pectin (P). Different nanoliposomal systems, i.e. NH-loaded nanoliposome (NH-NL), CH-coated NH-NL (CH-NH-NL), and P-coated CH-NH-NL (P-CH-NH-NL) were characterized through DLS, HPLC, TEM, and FTIR. The results confirmed good encapsulation efficiency (>90%) and successful layer formation with nano-sized and spherical carrier. Both CH-NL and P-CH-NL exhibited better physicochemical stability than NL under storage, thermal, pH, ionic, UV, oxidative, and serum conditions. In vitro mucin adsorption study revealed that CH-NL (60%) was more effective in mucoadhesion followed by P-CH-NL (46%) and NL (41%). Furthermore, P-CH-NL showed better performance in NH retention under different food simulants compared to CH-NH-NL and NH-NL, in which the release was mainly governed by the diffusion process. Thus, the P-CH conjugated nanoliposome could be a promising nano-carrier for neohesperidin.In the present study, the chemical composition of Morinda citrifolia essential oils was determined by gas chromatography-mass spectrometry and was found to contain several anti-cancer compounds including L-scopoletin, nordamnacanthal, β-morindone, α-copaene, 9-H-pyrido[3,4-b]indole, β-thujene and terpinolene. see more The physico-chemical characterization of chitosan, chitosan nanoparticles and Morinda citrifolia essential oils loaded chitosan nanoparticles combination was carried out by Fourier transform infrared spectroscopy, powder X-ray diffraction and dynamic light scattering coupled with zeta potential. The morphological observation obtained by scanning electron microscopy and transmission electron microscopy provided clear indication that the immobile chitosan polymer formed a coating onto the Morinda citrifolia essential oils surface. The cytotoxic effect of Morinda citrifolia essential oils loaded chitosan nanoparticles against A549 cells were investigated, resulting in 54% inhibition at 40 μg/ml-1. Information about in vitro morphological modification, nucleus damages, ROS generation and cell cycle arrest was obtained by fluorescence microscopy and flow cytometer analysis. The toxicity evaluation against human red blood cells suggested that the Morinda citrifolia essential oils loaded chitosan nanoparticles possess minimum cytotoxicity. Altogether, the present study suggests that these Morinda citrifolia essential oils loaded chitosan nanoparticles are valuable biomaterials owing to their ability to fight against A549 cancer cells.Heavy metal ions pollution is a terrible issue that needs to be efficiently treated as a matter of priority to construct our sustainable society. However, the easy-to-handling of high-performance biomass-derived sorbents with fascinating features like high sorption capacity, favorable separation and recycling remain challenging. Herein, the development of a novel bead-like adsorbent with above features, that is, Al(III)-assembled carboxymethyl cellulose beads were used for the removal of Pb(II), Ni(II) and Co(II) from aqueous solution. Characterization methods like FT-IR, SEM, XPS and TGA were employed to confirm its physicochemical properties. Removal of the three heavy metal ions at different pH values, initial concentration and contact time were discussed at batch adsorption experiments. Meanwhile, regeneration was also discussed deeply. The results revealed that the adsorption capacity of the sorbents for three heavy metals increases with increasing pH and the initial concentration. The adsorption isotherm could be described well by the Freundlich model, and the maximum adsorption capacity for Pb(II), Ni(II) and Co(II) were 550, 620 and 760 mg/g, respectively. Kinetics study indicated that the Pseudo-