Sheehan Waters (coilspace74)
Arbuscular mycorrhizal fungi (AMFs) play a vital role in ecosystems, especially in ecosystem variability, diversity, and function. Understanding the AMF diversity, distribution, and their driver at different altitudinal gradients is a benefit for understanding the ecological function of AMF in mountain ecosystems. In this study, we explored the AMF molecular diversity and their distribution from 660 to 3,500 m a.s.l. in Mount Taibai of Qinling Mountains based on high-throughput sequencing technology. A total of 702 operational taxonomic units (OTUs) in 103 species of AMF are isolated from soil samples, which belong to 18 identified and 1 unidentified genus in 10 families. The fungi in the genus of Glomus is the most dominant, with the occurrence frequency of 100% and the relative abundance of 42.268% and 33.048% on the species and OTU level, respectively. The AMF colonization in root could be simulated by a cubic function with the change of altitudes with the peak and trough at a.s.l. 1,170 and 2,850 m, respectively. Further, AMF diversity indices including Sob, Shannon diversity, and Pielou evenness also showed the same cubic function change trends with increasing altitude at OTU and species levels. However, the average values of diversity indices at OTU level are always higher than these at the species level. Based on the OTU level, the highest and lowest values of Shannon and Pielou indices are observed at the altitudes of 1,400 and 2,800 m, respectively. The pattern of AMF community distribution in Mt. Taibai is driven by altitude with the characteristics of more abundance in the medium- to low-altitude than high-altitude areas. In general, abundant AMF molecular diversity and species exit in different elevations of Mt. Taibai, which indicate gradient changes with elevations.Blogging is a widely used social medium for storing and sharing information online. Being an attractive online interface, some studies show that education blogging or edublogging might promote more engaged learning. An apathy to contemporary issues related to one's area of study can result in a less knowledgeable student who is less ready for the job industry. To bridge the gap between classroom learning and awareness of emerging issues pertaining to the field of study and potential employment - blogging of ongoing events in a select microbiological field was proposed as a graded semester-long activity called "Disease Tracking." The exercise involved instructing students to choose one infectious disease topic, for which traditional and non-traditional scientific information could be sourced with high frequency over the preceding months. Students were to document new information on the topic as it became available over the term, from reliable information resources. At the end of the term, students presented their work in a "Blog show-off" presentation session. Blog-based learning was found to be an engaging tool that satisfied all criteria under Bloom's taxonomy. UNC0638 concentration Students developed a continued intrigue for the chosen topic and appreciated the diverse fields in which fundamentals of infectious diseases learned in class, could be applied within and outside academia. Students also valued this experience and feedback showed that the freedom to choose their own topic (77%), opportunity to learn more from other students' blogs (77%), less stress as they were not competing on identical topics (73%), a "fun way" to learn (68%), and an opportunity to understand the importance of staying abreast with scientific news (64%) stood out as the chief positive points of the exercise to the students. In view of these benefits, blogs can be used for an immersive, broad learning experience in Microbiology and other fields in which there is likely to be a frequency of new information online.Outer membrane vesicles (OMVs) from Gram-negative bacteria were first described more than 50 years ago. However, the molecular mechanisms involved in biogenesis