Panduro Boisen (cartank1)
On the basis of our results, we assume that even in long-term rehabilitation of severely impaired non-fluent aphasia patients the applied treatment may support coactivation with dorsolateral prefrontal regions, suggested to be particularly involved in cognitive processing. This left-lateralized dorsolateral prefrontal-parietal network is supposed to be engaged in domain-general aspects of active phonological memory. To the best of our knowledge, no comparable studies are available as yet. Therefore, we hope that our study may serve to attract more attention for the late stages of long-term rehabilitation, not at least as a challenge for therapists and researchers alike.Speech perception under "cocktail-party" conditions critically depends on the focusing of attention toward the talker of interest. In dynamic auditory scenes, changes in talker settings require rapid shifts of attention, which is especially relevant when the position of a target talker switches from one location to another. Here, we explored electrophysiological correlates of shifts in spatial auditory attention, using a free-field speech perception task, in which sequences of short words (a company name, followed by a numeric value, e.g., "Bosch-6") were presented in the participants' left and right horizontal plane. Younger and older participants responded to the value of a pre-defined target company, while ignoring three simultaneously presented pairs of concurrent company names and values from different locations. All four stimulus pairs were spoken by different talkers, alternating from trial-to-trial. The location of the target company was within either the left or right hemisphere for a variable numbernd alpha lateralization reflect different attentional processes in multi-talker speech perception, the first being primarily associated with auditory search and the focusing of attention, and the second with the in-depth attentional processing of task-relevant information. Especially the second process appears to be prone to age-related cognitive decline.Combination of complexes of lanthanide cations (Ln3+) for their luminescent properties and peptides for their recognition properties is interesting in view of designing responsive luminescent probes. The octadentate DOTA (1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid) chelate is the most popular chelate to design Ln3+ complex-peptide conjugates. We describe a novel building block, DO3Apic-tris(allyl)ester, which provides access to peptides with a conjugated nonadentate chelate, namely DO3Apic, featuring a picolinate arm in place of one of the acetate arms compared to DOTA, for improved luminescence properties. This building block, with allyl protecting groups, is readily obtained by solid phase synthesis. We show that it is superior to its analogue with tBu protecting groups for the preparation of peptide conjugates because of the difficult removal of the tBu protecting groups for the latter. Then, we compare two luminescent zinc fingers (LZF) comprising (i) a zinc finger peptide for selective Zn2+ binding, (ii) a Eu3+ complex and (iii) an acridone antenna (ACD) for long-wavelength sensitization of Eu3+ luminescence. The first one, LZF3ACD|Eu, incorporates a DOTA chelate for Eu3+ whereas the other, LZF4ACD|Eu, incorporates a DO3Apic chelate. Both act as Zn2+-responsive luminescent probes but we show that changing DOTA for DO3Apic results in a higher Eu3+ luminescence lifetime and in a doubling of the quantum yield, confirming the interest of the DO3Apic chelate and the DO3Apic(tris(allyl)ester building block for the preparation of Ln3+ complex-peptide conjugates. Additionally, the DO3Apic chelate provides self-calibration for LZF4ACD|Eu luminescence upon excitation of its picolinamide chromophore, making LZF4ACD|Eu a ratiometric sensor for Zn2+.Accurate prediction of the subsurface transport of iodine species is important for the assessment of long-term nuclear waste repository performance, as w
