Cobb Knowles (caremiddle35)

3% and 24.5%, respectively, with hookworms being the most detected parasites in dogs (8.3%) and Dipylidium caninum in cats (12.5%). Sex was the only variable that showed statistical differences in the canine population males being more often infected than female (p less then 0.05), while street access was associated with parasitic infections in cats (p less then 0.05). The results obtained in this study demonstrate the need to control parasitic infections in domiciled dogs and cats in different municipalities in the metropolitan region of the state of Rio de Janeiro, including residents in the northern, western and central areas.The utility of mitochondrial cytochrome oxidase subunit I (COX1) and 16S ribosomal DNA (16S-rDNA) sequence analyses as a complementary/alternative tool to classical taxonomy, for the identification of some of the most prevalent hard tick species from Portugal was evaluated using BOLD-ID (COX1 only), BLASTn and phylogenetic tree reconstruction based on multiple nucleotide sequence alignments. Both molecular markers proved suitable for identifying ticks to a species level, but specific aspects that limit their resolving power must be considered. Their accuracy of tick identification in all life stages and of the other tick species described in the South of Europe is required.Anaplasma marginale infection in cattle (n = 216) in the states of Uttar Pradesh and Uttarakhand, North India was screened by microscopy and nested-polymerase chain reaction (PCR). Two recombinant proteins viz. major surface protein (MSP) 5 and MSP2 of A. marginale were expressed in Escherichia coli and their potential in the detection of antibodies to Anaplasma species in the cattle was evaluated by immunoglobulin G-enzyme linked immunosorbent assay (IgG-ELISA). The MSP5 IgG ELISA results were compared with competitive (c) inhibition ELISA. Microscopy being the least sensitive diagnostic test detected 12.0% of animals positive for A. marginale infection while nested-PCR detected 87.9% of these animals as positive for A. marginale infection. The recombinant MSP5 antigen showed positive reactivity in 170/190 nested-PCR confirmed positive animals (sensitivity 89.5%) with specificity of 77.0%. In comparison, the recombinant MSP2 antigen showed lesser sensitivity and specificity of 79.0% and 69.2%, respectively. The cELISA was more sensitive and specific than IgG-ELISA. However, molecular detection by msp5 nested-PCR was highly sensitive and reliable for detection of carrier cattle for Anaplasma infection. The study indicated that a large cattle population (87.9%) was carrier for A. marginale infection in this region of the country.The present study explored the frequency of hair-sheep farms with gastrointestinal nematodes (GIN) resistant to albendazole sulfoxide (AS), ivermectin (IVM) and levamisole (LEV) in the Yucatán peninsula, México, using the faecal egg count reduction test (FECRT), and compared the frequency of farms diagnosed with resistance using three different formulae. The survey included farms from the states of Campeche (9) and Yucatán (14) (2016-2019). Collaborating farms had >100 grazing ewes. Animals in the FECRT were > 12 months old, received no anthelmintic for >8 weeks and had ≥150 GIN eggs per gramme of faeces (EPG). Animals were distributed to respective groups untreated controls, AS (5 mg/kg BW), IVM (0.2 mg/kg BW), and LEV (7.5 mg/kg BW). Due to low EPG, some farms only included one or two AH groups. Second faecal samples were obtained on day 14 post-treatment to estimate the percentage reduction (%R) and 95% confidence interval (95%CI). Criteria to declare resistance were those proposed by the World Association cannot identify what proportion of the %R cannot be attributed to the AH. The untreated control group helped adjusting the %R calculation and seemed more adequate considering the propensity of hair-sheep to reduce their EPG on their own. .Small ruminant fasciolosis (SRF) is a snail - borne dise