Ryberg Gillespie (carehyena0)
Loss of membrane potential of sperm mitochondria has been regarded as the first step preceding mitophagy degradation after their entry into the C. elegans oocyte at fertilization. This is in line with the classical view of mitophagy of defective or abnormal mitochondria and could serve as a recognition signal for their specific and quick autophagy degradation. Here, using TMRE (tetramethylrhodamine ethyl ester) and live imaging we show that this is not the case. Instead, sperm inherited mitochondria show a stable labeling with TMRE before and at the time of autophagosomes formation. Interestingly, this labeling remains in late-stage-embryos of autophagy-defective-mutants suggesting that the loss of membrane potential occurs upon the entry of the mitochondria into the autophagy pathway. These stabilized and still polarized sperm mitochondria remain distinct but associated with the maternal-derived mitochondrial network suggesting a mechanism that prevents their fusion and represents an efficient additional protective system against fertilization-induced heteroplasmy.Electronic devices play vital role in modern civilization. Compared to conventional electronic manufacturing, the recently emerging liquid metal printed electronics (LMPE) is opening many extraordinary opportunities, such as large-area printing, pervasive adaptability, flexibility for personal use, low cost, high performance, and environmental friendliness. More uniquely, liquid metal printing allows customize electronic products on demand to fabricate electronics spanning from 2D plane surface to 3D structure and on any desired substrates. This deems it to reshape modern electronics and integrated circuits field. So far, a variety of technological breakthroughs in this new generation electronic engineering area have been made in the process of developing various liquid metal functional inks, printing machines and applications, which significantly stimulate the quick incubation and formation of a new electronic industry. Clearly, sorting out the major R&D directions and clarifying future challenges is crucial for the large scale industrialization of LMPE. This perspective article is dedicated to briefly outline the representative principles and key technologies lying behind, and illustrate the milestone products and equipment thus invented for the coming LMPE industry. In addition, we evaluate the corresponding industrialization trends and promising roadmap and interpret future prospects for the new era of pervasive electronics when anyone can freely use such a tool to print out himself functional electronic device to fulfill various purposes at anywhere and anytime.We resolve debate over the evolution of vertebrate hypermineralized tissues through analyses of matrix protein-encoding secretory calcium-binding phosphoprotein (SCPP) genes and phylogenetic inference of hypermineralized tissues. Among these genes, AMBN and ENAM are found in both sarcopterygians and actinopterygians, whereas AMEL and SCPP5 are found only in sarcopterygians and actinopterygians, respectively. Actinopterygian AMBN, ENAM, and SCPP5 are expressed during the formation of hypermineralized tissues on scales and teeth ganoin, acrodin, and collar enamel in gar, and acrodin and collar enameloid in zebrafish. Our phylogenetic analyses indicate the emergence of an ancestral enamel in stem-osteichthyans, whereas ganoin emerged in stem-actinopterygians and true enamel in stem-sarcopterygians. Thus, AMBN and ENAM originated in concert with ancestral enamel, SCPP5 evolved in association with ganoin, and AMEL evolved with true enamel. Shifts in gene expression domain and timing explain the evolution of different hypermineralized tissues. We propose that hypermineralized tissues in osteichthyans coevolved with matrix SCPP genes.Chaperonins play an important role in folding newly synthesized or translocated proteins in all organisms. The bacterial chaperonin GroEL has served as a model system for the
