Ernst Batchelor (capcycle26)

The aims were to investigate the prevalence of intramammary infection in early-lactation primiparous dairy cows (PC) in Sweden using milk recording cow composite somatic cell count (CSCC) categories based on classification of CSCC at the first 2 milk recordings after calving as low or high using cut-offs indicating intramammary infection. We also wanted to evaluate if herd-specific patterns in CSCC categories among PC can be identified to indicate success and problem herds as well as cow-level associations between CSCC categories and breed and sire, and herd-level associations between CSCC categories and herd size, milk production, production system, milking system, and year. A total of 1,597 dairy herds were included. Inclusion criteria were enrolment in the Swedish official milk recording scheme and having production data from at least 10 PC per year during 2014, 2015, and 2016. Herd (size, SCC, milk production, production system, milking system) and cow (breed, lactation number, calving date, CSCC, milk prubclinical mastitis in early-lactation PC as only 50% of these cows had low CSCC at both milk recordings after calving. Moreover, we conclude that CSCC categories may be a useful tool for identifying success and problem herds.Metabolic fates of fatty acids in tissue may be influenced by extracellular concentration and profile of fatty acids. Previous work has demonstrated the ability of C183n-3 cis to ameliorate the effects of C160- or C180-induced depression of pyruvate carboxylase (PC) mRNA expression. Pyruvate carboxylase catalyzes oxaloacetate synthesis and connects gluconeogenesis from lactate and fatty acid metabolism. selleckchem Our objective was to determine the effects of co-presence of saturated and unsaturated fatty acids on cellular partitioning of [1-14C]C160 metabolism to CO2 or acid-soluble products (ASP) in Madin-Darby bovine kidney cells and the role of PC in this relationship. We hypothesized that the ratio of saturated to unsaturated fatty acid pretreatments regulates [1-14C]C160 partitioning to CO2 or ASP. Cells were exposed for 21 h to either individual fatty acids, C160, C180, C181n-9 cis, or C183n-3 cis, or to fatty acid combinations in 1090, 2575, 5050, 7525, or 9010 ratios for 3 combinations C160/C183n-3 cis, C180/C1orrelations were determined for PC mRNA expression and rate of [1-14C]C160 metabolism to CO2 or ASP, including ketones, and for PCK1 mRNA expression and rate of [1-14C]C160 metabolism to CO2 or ASP. Production of CO2 from [1-14C]C160 was positively correlated (r = 0.63) with PC expression, whereas ASP production from [1-14C]C160 only tended to positively correlate (r = 0.51) with PC mRNA expression. Production of CO2 or ASP from [1-14C]C160 were both positively correlated (r = 0.80 and r = 0.69, respectively) with PCK1 expression. Results show a regulation of ketone production by Madin-Darby bovine kidney cells in response to saturated and unsaturated fatty acid pretreatments.In-depth analysis of colostrum components has identified hundreds of proteins, but data are sparse regarding their systemic uptake in the newborn calf. Moreover, heat treatment may influence these colostral components and their absorption. Our objectives were to describe the serum proteome of newborn calves before and after colostrum feeding and the possible effects of colostral heat treatment. Newborn Holstein heifer calves (n = 22) were randomized within pair and fed heat-treated (n = 11; 60°C, 60 min) or raw (n = 11) colostrum at 8.5% of birth body weight by esophageal feeder within 1 h of birth. After the single colostrum feeding, calves were not fed until after the 8-h time point, when milk was offered free-choice. Blood samples were taken immediately before feeding (0 h), as well as 4, 8, and 24 h after feeding. Whole blood packed cell volume (%), serum Brix percentage, and plasma glucose concentrations were determined for all time points. Plasma insulin and insulin-like growth factor-I concentrations we