Nymann Fuglsang (brazilmove89)

This differentiates the spun-in enzymes from most of the matrix-enveloped enzymes, which are molecularly dispersed in the matrix. On <a href=";>;/a> , the fiber material need to be so dense that the enzymes are retained in it and not "bleed out". On the other hand, the material ought to have sufficient pores to enable substrates and items to diffuse in and out without important hindrance. <iframe width="400" height="225" src="; frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture" allowfullscreen></iframe>In <a href=";>;/a> , spun-in enzymes are not suitable for converting high-molecular substrates such as protein or starch. All spinning strategies employed so far begin with the solution of an organic polymer appropriate for fiber formation, which is insoluble in water, in a water-immiscible organic solvent. Aqueous dissolved enzymes or suspended cells are emulsified into the organically dissolved polymer. The emulsion is drawn from a fine nozzle by way of a precipitation bath, the polymer becoming hardened in fiber type. A whole series of diverse enzymes could already be effectively immobilized by spinning. <img src=";/> So far, on the other hand, only spun-in yeast-β-galactosidase has achieved industrial significance (see Chapter 5. 7, p. 110f). So far, this has been the most important explanation for employing spun-in ß-galactosidase from yeast. ß-galactosidase from molds was already immobilized in job four by cross-linking (see p. 160ff). This corresponds to the pH range of sour whey, which falls off in huge quantities throughout cheese production. The yeast ß-galactosidase employed right here, on the other hand, has its optimum at pH 6 to six.5 and is as a result especially appropriate for the therapy of milk that has comparable pH values. The glucose content of the heat-inactivated sample is determined applying the hexakinase technique (glucose test combination No. <a href=";>;/a> , Boehringer Mannheim GmbH, D-6800 Mannheim). The glucose concentration in the blank sample is also determined. The spinning of ß-galactosidase from yeast, which is also important industrially to a limited extent, serves as an example. When immobilized by spinning, the enzymes dissolved in water are enclosed as droplets in the microcaverns of semipermeable fibers. To establish the activity of the soluble enzyme, 20 mg glucoamylase are dissolved in 50 ml with .1 M citrate buffer and made use of in the test mixture. It serves as a reference point for observations of activity and yield. A 3.3 Results and evaluation Table 32 shows the measurement and calculation benefits of a common series of tests. If the activity values identified in the immobilizates and supernatants of the individual test variants are added collectively, the outcome is sums among 9.S33 and .mol / g min (units / g).