Norwood McCain (baconship8)

The splenocytes conditioned media cultured with GSF3 increased Fas mRNA expression amounts in the treated MCF-7 cells. There was a significant negative correlation between Th2-polarized cytokines secreted by immune cells and Fas mRNA expression levels in the corresponding treated MCF-7 cells. N-Acetyl-DL-methionine chemical structure Our findings suggested that GSF3 is a potent anti-cancerous polysaccharide by direct action or indirectly modulating immune cell cytokine secretion profiles.Incorporation of drugs in clay minerals has been widely proposed for the controlled-release or increased solubility of drugs. In this context, a bionanocomposite based on kaolinite and cashew gum (Kln/Gum) was synthesized and characterized by X-ray diffraction (XRD), thermal analysis (TG/DTA), and Fourier transform infrared spectroscopy (FTIR). The bionanocomposite was applied to the incorporation and further release of doxazosin mesylate (DB). The influence of solution pH (1-3), adsorbent dose (20-50 mg), initial drug concentration (20.0-70.0 mg L-1), contact time (15-300 min), and temperature (25, 35, and 45 °C) were systematically evaluated. Equilibrium was reached around 60 min, with a maximum adsorption capacity of 31.5 ± 2.0 mg g-1 at a pH of 3.0 and 25 °C. Hydrogen bonding contributed to DB incorporation on the Kln/Gum. In addition, DB maximum amounts of 16.80 ± 0.58 and 77.00 ± 2.46% were released at pH values of 1.2 and 7.4, respectively. These results indicated that the Kln/Gum bionanocomposite is an effective and promising material for the incorporation/release of drugs with similar structures to DB.The CONSTANS-like (COL) genes play an important role in the photoperiodic flowering pathway. Poplar is a perennial woody plant with a long juvenile phase, but the molecular characterization of COL genes in Populus is limited. In this study, 14 COL genes were identified in the Populus genome. Phylogenetic analysis indicated the PtCOL proteins were divided into three subgroups, and the members of each subgroup had similar gene structure and motif composition. Chromosome distribution analysis showed that 14 PtCOL genes were distributed on 10 chromosomes. Multiple sequence alignment indicated that these proteins contained a highly conserved B-box1 and a conserved CCT domain, but the B-box2 structure was divided into three different types. Promoter analysis found that there were several light-responsive cis-elements in the PtCOL genes. Furthermore, tissue-specific expression showed that all nine PtCOL genes were widely expressed in various tissues and organs of Populus, and were preferentially expressed in the leaves. Additionally, the transcription level of PtCOL exhibited a diurnal oscillation pattern in different light conditions. This study not only provided comprehensive information for further analysis of the function of the PtCOL gene family, but also revealed the biological roles of PtCOL genes in the photoperiod-dependent flowering process of Populus.Lignin-carbohydrate complex (LCC) was proven to possess antioxidant activities. To understand structures and antioxidant activities of LCC, water-soluble LCC-stalk, LCC-sheath and LCC-leaf were isolated from ball-milled wheat straw stalk, sheath and leaf by successive dissolution in LiCl/DMSO solvent, water extraction and purification. LCCs were later structurally characterized by wet chemistry, chromatography and spectroscopy respectively. Their antioxidant activities were evaluated by ferric reducing antioxidant power (FRAP) and DPPH radical scavenging assays. The results showed that three LCCs were carbohydrate-rich (≈70%) and presented relatively narrow molecular weight distribution (PI less then 2.0). The lignin moieties of LCCs were mainly connected with β-O-4' structures, and phenyl glycoside and γ-ester linkages were main LCC linkages in LCCs. However, guaiacyl units were the predominant lignin units in LCC-stalk and LCC-sheath, while syringyl units were predominant in LCC-le